Anti-STAT5a (phospho Y694) antibody (ab30648)
Key features and details
- Rabbit polyclonal to STAT5a (phospho Y694)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-STAT5a (phospho Y694) antibody
See all STAT5a primary antibodies -
Description
Rabbit polyclonal to STAT5a (phospho Y694) -
Host species
Rabbit -
Specificity
ab30648 detects endogenous levels of STAT5A only when phosphorylated at Tyrosine 694. Antibody ab31377 is raised against the same peptide sequence but non-phosphorylated at tyrosine 694. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic phosphopeptide derived from human STAT5a around the phosphorylation site of Tyrosine 694.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5a (phospho Y694) antibody (ab30648)Immunohistochemical analysis of paraffin-embedded breast carcinoma tissue. Left: Using ab30648 (10ug/ml); right: the same antibody preincubated with synthesized phosphopeptide.
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Western blot - Anti-STAT5a (phospho Y694) antibody (ab30648)All lanes : Anti-STAT5a (phospho Y694) antibody (ab30648)
Lane 1 : HeLa cells untreated
Lane 2 : HeLa cells treated with EGF
Predicted band size: 91 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
