Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - ChIP Grade – BSA and Azide free (ab277618)
![Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - ChIP Grade – BSA and Azide free (ab277618)](https://www.abcam.com/ps/products/277/ab277618/Images/ab277618-415164-anti-stat2-phospho-y690-antibody-epr1854942-chip-a431-cells.JPG "Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - ChIP Grade – BSA and Azide free (ab277618)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18549-42] to STAT2 (phospho Y690) - ChIP Grade – BSA and Azide free
- Suitable for: WB, IP, Dot blot, ChIP
- Reacts with: Human
Overview
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Product name
Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - ChIP Grade – BSA and Azide free
See all STAT2 primary antibodies -
Description
Rabbit monoclonal [EPR18549-42] to STAT2 (phospho Y690) - ChIP Grade – BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, Dot blot, ChIPmore details
Unsuitable for: Flow Cyt,ICC or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A431 (starved overnight, then 10 ng/ml IFN-alpha treated for 30 minutes) whole cell lysate. IP: K562 (treated with 2500 U/ml IFN alpha for 30 mins) whole cell lysate. ChIP: Chromatin prepared from A431 cells (starved overnight, treated with IFN-alpha (10ng/ml 0.5 h).
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General notes
ab277618 is the carrier-free version of ab191601. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab277618 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18549-42 -
Isotype
IgG -
Research areas
Images
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ChIP - Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - BSA and Azide free (ab277618)
This data was developed using ab191601, the same antibody clone in a different buffer formulation.
Chromatin was prepared from A431(human epidermoid carcinoma cell line)(starved overnight, treated with IFN-α (10ng/ml 0.5 h)) and A431 (starved overnight) (non-treated) according to the Abcam Dual-X-ChIP protocol*. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
The ChIP was performed with 25 µg of chromatin, 5 µg of ab191601 (Red), or 5 µg of rabbit normal IgG ab172730 (Gray) and 25 µl of Protein A/G Dynabeads. The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).Primers are from paper PMID:30240626.
*https://www.abcam.com/resources?keywords=X%20ChIP%20protocol
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Western blot - Anti-STAT2 (phospho Y690) antibody - BSA and Azide free (ab277618)All lanes : Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - ChIP Grade (ab191601) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma epithelial cell) (starved overnight, then 10 ng/ml IFN-alpha treated for 30 minutes), whole cell lysate
Lane 2 : A431 (starved overnight), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 97 kDa
Observed band size: 113,97 kDa why is the actual band size different from the predicted?This data was developed using ab191601, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 123 seconds.
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![Immunoprecipitation - Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - BSA and Azide free (ab277618)](https://www.abcam.com/ps/products/277/ab277618/Images/ab277618-415166-anti-stat2-phospho-y690-antibody-epr1854942-immunoprecipitation-k562.jpg)
Immunoprecipitation - Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - BSA and Azide free (ab277618)This data was developed using ab191601, the same antibody clone in a different buffer formulation.
STAT2 (phospho Y690) was immunoprecipitated from 0.35 mg K-562 (human chronic myelogenous leukemia) (treated with 2500 U/ml IFN alpha for 30 mins) whole cell lysate with ab191601 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab191601 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/1000 dilution.
Lane 1: K-562 (human chronic myelogenous leukemia) (treated as above) whole cell lysate 10 ug
Lane 2: ab191601 IP in K-562 (treated as above) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab191601 in K-562 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
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![Dot Blot - Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - BSA and Azide free (ab277618)](https://www.abcam.com/ps/products/277/ab277618/Images/ab277618-415163-anti-stat2-phospho-y690-antibody-epr1854942-dotblot-stat2.jpg)
Dot Blot - Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - BSA and Azide free (ab277618)This data was developed using ab191601, the same antibody clone in a different buffer formulation.
Dot blot analysis of STAT2 (phospho Y690) using ab191601 at 1/1000 dilution (1.4 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.
Lane 1: STAT2 (phospho Y690) phospho peptide
Lane 2: STAT2 non-phospho peptideExposure time: 30 seconds.
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![Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - ChIP Grade – BSA and Azide free (ab277618)](https://www.abcam.com/ps/products/277/ab277618/Images/ab277618-447363-benefitsofrecombinantantibodies.png)
Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - ChIP Grade – BSA and Azide free (ab277618)
