Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - ChIP Grade – BSA and Azide free (ab277618)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18549-42] to STAT2 (phospho Y690) - ChIP Grade – BSA and Azide free
  • Suitable for: WB, IP, Dot blot, ChIP
  • Reacts with: Human

Overview

  • Product name

    Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - ChIP Grade – BSA and Azide free
    See all STAT2 primary antibodies

  • Description

    Rabbit monoclonal [EPR18549-42] to STAT2 (phospho Y690) - ChIP Grade – BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, Dot blot, ChIPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A431 (starved overnight, then 10 ng/ml IFN-alpha treated for 30 minutes) whole cell lysate. IP: K562 (treated with 2500 U/ml IFN alpha for 30 mins) whole cell lysate. ChIP: Chromatin prepared from A431 cells (starved overnight, treated with IFN-alpha (10ng/ml 0.5 h).

  • General notes

    ab277618 is the carrier-free version of ab191601. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

     ab277618 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

     Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • ChIP - Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - BSA and Azide free (ab277618)
    ChIP - Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - BSA and Azide free (ab277618)

    This data was developed using ab191601, the same antibody clone in a different buffer formulation. 

    Chromatin was prepared from A431(human epidermoid carcinoma cell line)(starved overnight, treated with IFN-α (10ng/ml 0.5 h)) and A431 (starved overnight) (non-treated) according to the Abcam Dual-X-ChIP protocol*. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab191601 (Red), or 5 µg of rabbit normal IgG ab172730 (Gray) and 25 µl of Protein A/G Dynabeads. The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

    Primers are from paper PMID:30240626.

    *https://www.abcam.com/resources?keywords=X%20ChIP%20protocol

  • Western blot - Anti-STAT2 (phospho Y690) antibody - BSA and Azide free (ab277618)
    Western blot - Anti-STAT2 (phospho Y690) antibody - BSA and Azide free (ab277618)
    All lanes : Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - ChIP Grade (ab191601) at 1/1000 dilution

    Lane 1 : A431 (human epidermoid carcinoma epithelial cell) (starved overnight, then 10 ng/ml IFN-alpha treated for 30 minutes), whole cell lysate
    Lane 2 : A431 (starved overnight), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 97 kDa
    Observed band size: 113,97 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab191601, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 123 seconds.

     

  • Immunoprecipitation - Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - BSA and Azide free (ab277618)
    Immunoprecipitation - Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - BSA and Azide free (ab277618)

    This data was developed using ab191601, the same antibody clone in a different buffer formulation. 

    STAT2 (phospho Y690) was immunoprecipitated from 0.35 mg K-562 (human chronic myelogenous leukemia) (treated with 2500 U/ml IFN alpha for 30 mins) whole cell lysate with ab191601 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab191601 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/1000 dilution.

    Lane 1: K-562 (human chronic myelogenous leukemia) (treated as above) whole cell lysate 10 ug

    Lane 2: ab191601 IP in K-562 (treated as above) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab191601 in K-562 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Dot Blot - Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - BSA and Azide free (ab277618)
    Dot Blot - Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - BSA and Azide free (ab277618)

    This data was developed using ab191601, the same antibody clone in a different buffer formulation. 

    Dot blot analysis of STAT2 (phospho Y690) using ab191601 at 1/1000 dilution (1.4 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.

    Lane 1: STAT2 (phospho Y690) phospho peptide
    Lane 2: STAT2 non-phospho peptide

    Exposure time: 30 seconds.

  • Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - ChIP Grade – BSA and Azide free (ab277618)
    Anti-STAT2 (phospho Y690) antibody [EPR18549-42] - ChIP Grade – BSA and Azide free (ab277618)

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