Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3146] to STAT1 (phospho S727)
  • Suitable for: WB, IHC-P, Dot blot
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-STAT1 (phospho S727) antibody [EPR3146]
    See all STAT1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3146] to STAT1 (phospho S727)

  • Host species

    Rabbit

  • Specificity

    A phospho specific peptide corresponding to residues surrounding Serine 727 of human Stat-1 was used as an immunogen. This antibody only detects Stat-1 phosphorylated at Serine 727.

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa cell lysate; Human stomach adenocarcinoma tissue

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461)
    Western blot - Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461)
    All lanes : Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461) at 1/5000 dilution (purified)

    Lane 1 : Untreated HeLa whole cell lysate
    Lane 2 : HeLa whole cell lysate treated with etoposide
    Lane 3 : HeLa whole cell lysate treated with etoposide, followed by membrane treatment with phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 87 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 2% BSA/TBST
    Dilution buffer: 2% BSA/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461)
    Immunohistochemical staining of paraffin embedded rat colon with purified ab109461 at a working dilution of 1/200. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461)
    Immunohistochemical staining of paraffin embedded mouse colon with purified ab109461 at a working dilution of 1/200. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461)
    Immunohistochemical staining of paraffin embedded human breast carcinoma with purified ab109461 at a working dilution of 1/200. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Western blot - Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461)
    Western blot - Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461)
    All lanes : Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461) at 1/10000 dilution (unpurified)

    Lane 1 : Untreated HeLa (human cervix adenocarcinoma)
    Lane 2 : HeLa (human cervix adenocarcinoma) membrane treated with Alkaline Phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1500 dilution

    Developed using the ECL technique.

    Predicted band size: 87 kDa
    Observed band size: 91 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    The lower section shows STAT1 detected with ab92506, anti-STAT1 antibody, to confirm that the same amount of lysate is used in each lane.

    Blocking and dilution biffer: 5% NFDM/TBST.

  • Dot Blot - Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461)
    Dot Blot - Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461)

    Dot Blot analysis of Lane 1: STAT1 (pS727) phospho peptide and Lane 2: STAT1 non-phospho peptide, labeling STAT1 (phospho S727) with ab109461 at 1/1000 dilution. 5% NFDM/TBST was used as the blocking and diluting buffer. ab97051, a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody was used at 1/100000 dilution. Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461)

    Unpurified ab109461, at a 1/100 dilution, staining STAT1 (phospho S727) in paraffin embedded Human stomach adenocarcinoma tissue by Immunohistochemistry.

  • Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461)
    Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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