Anti-STAT1 (phospho Y701) antibody (ab30645)
Key features and details
- Rabbit polyclonal to STAT1 (phospho Y701)
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-STAT1 (phospho Y701) antibody
See all STAT1 primary antibodies -
Description
Rabbit polyclonal to STAT1 (phospho Y701) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic phosphopeptide derived from human STAT1 around the phosphorylation site of Tyrosine 701.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-STAT1 (phospho Y701) antibody (ab30645)All lanes : Anti-STAT1 (phospho Y701) antibody (ab30645)
Lane 1 : Untreated MCF7 lysate (5-30ug).
Lane 2 : EGF treated MCF7 lysate (5-30ug).
Predicted band size: 87 kDa
Observed band size: 87 kDa
Additional bands at: 47 kDa. We are unsure as to the identity of these extra bands.
Suggested dilution: 1:500 - 1:1000 -
Immunocytochemistry/ Immunofluorescence - Anti-STAT1 (phospho Y701) antibody (ab30645)ICC/IF image of ab30645 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30645, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 (phospho Y701) antibody (ab30645)Paraffin-embedded human breast carcinoma tissue stained for STAT1 (phospho Y701) using ab30645 at 1/590 dilution in immunohistochemical analysis. Tissue was incubated in the absence (left) or presence (right) of immunizing phospho-peptide.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 (phospho Y701) antibody (ab30645) This image is courtesy of an anonymous Abreviewab30645 staining STAT1 (phospho Y701) in murine intestine tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 23°C; antigen retrieval was by heat mediation with a citrate buffer. Samples were incubated with primary antibody (1/400 in Tris Buffer Saline) for 16 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/200) was used as the secondary antibody.
