Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22599-52] to STAT6 (phospho Y641) - BSA and Azide free
  • Suitable for: ChIP, IP, Dot blot, Flow Cyt (Intra), WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free
    See all STAT6 primary antibodies

  • Description

    Rabbit monoclonal [EPR22599-52] to STAT6 (phospho Y641) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ChIP, IP, Dot blot, Flow Cyt (Intra), WBmore details
    Unsuitable for: ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB and IP: Daudi serum starved for 24 hours, then treated with 100 ng/ml IL-4 for 15 minutes, whole cell lysate; 2.4G2 serum starved for 24 hours, then treated with 100 ng/ml rIL-4 for 15 minutes, whole cell lysate; RAW264.7 serum starved for 24 hours, then treated with 100 ng/ml mIL-4 for 15 minutes, whole cell lysate. Flow Cyt (intra): Daudi was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min; RAW 264.7 was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min

  • General notes

    ab263949 is the carrier-free version of ab235591.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • ChIP - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949)
    ChIP - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949)

    Chromatin was prepared from Daudi cells starved for 24h, then treated with hIL-4(100ng/ml, 15min) according to the Abcam Dual-X-ChIP protocol*. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min. 
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab235591 (red), or 5 µg of rabbit normal IgG ab172730 (gray) and 20 µl of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). 
    Primers and probes are commercial primers from Cell Signaling Technology (Cat. No.: #56554) 
    *https://www.abcam.com/resources?keywords=X%20ChIP%20protocol

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235591).

  • Dot Blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949)
    Dot Blot - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949)

    Dot blot analysis using ab235591 at 1/1000 dilution, Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at 1/100,000 diluton.

    Lane 1: STAT6 (phospho Y641) peptide (aa636-647).

    Lane 2: STAT6 non-phospho peptide (aa636-647)

    Exposure time: 26 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235591).

  • Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949)
    Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949)

    STAT6 (phospho Y641) was immunoprecipitated from 0.35 mg RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate with ab235591 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab235591 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: RAW 264.7 cells serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate 10ug

    Lane 2: ab235591 IP in RAW 264.7 cells serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab235591 in RAW 264.7 was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235591).

  • Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949)
    Immunoprecipitation - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949)

    STAT6 (phospho Y641) was immunoprecipitated from 0.35 mg Daudi (Human Burkitt's lymphoma lymphoblast) cells serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate, with ab235591 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab235591 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Daudi cells serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate 10ug

    Lane 2: ab235591 IP in Daudi was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab235591 in Daudi cells serum starved for 24h, then treated with 100ng/ml IL-4 for 15min whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235591).

  • Flow Cytometry - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949)
    Flow Cytometry - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min (Red) / Untreated control (Green) cells labelling STAT6 (phospho Y641) with ab235591 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1:2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235591).

  • Flow Cytometry - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949)
    Flow Cytometry - Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Daudi (Human Burkitt's lymphoma lymphoblast) was serum starved for 24h, then treated with 100ng/ml IL-4 for 15min (Red) / Untreated control (Green) cells labelling STAT6 (phospho Y641) with ab235591 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1:2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235591).

  • Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949)
    Anti-STAT6 (phospho Y641) antibody [EPR22599-52] - BSA and Azide free (ab263949)

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