Antibodies, Proteins, Kits and Reagents for Life Science

Anti-STAT5 (phospho Y694) antibody [Stat5Y694-G11] (ab278764)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [Stat5Y694-G11] to STAT5 (phospho Y694)
Suitable for: WB, Flow Cyt
Reacts with: Mouse, Human

Product name

Anti-STAT5 (phospho Y694) antibody [Stat5Y694-G11]
See all STAT5 primary antibodies
Description

Rabbit monoclonal [Stat5Y694-G11] to STAT5 (phospho Y694)
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide within Human STAT5 (phospho Y694). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P42229

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Positive control
- WB: K562 cell extract. Flow cyt: NIH/3T3 and K562 cells (both treated with pervanadate)
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
Flow Cyt	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Concentration information loading...
Purity

Protein A/G purified
Clonality

Monoclonal
Clone number

Stat5Y694-G11
Isotype

IgG
Light chain type

kappa
Research areas

Flow Cytometry - Anti-STAT5 (phospho Y694) antibody [Stat5Y694-G11] (ab278764)

Flow cytometric analysis of K562 cells secondary antibody only negative control (blue) or untreated (grey) or treated with pervanadate (orange) using 0.1 µg/mL isotype control or untreated (red) or pervanadate (green) using ab278764.
Western blot - Anti-STAT5 (phospho Y694) antibody [Stat5Y694-G11] (ab278764)

All lanes : Anti-STAT5 (phospho Y694) antibody [Stat5Y694-G11] (ab278764) at 0.01 µg/ml

Lane 1 : K562 cell extract untreated
Lane 2 : K562 cell extract treated with imatinib

Predicted band size: 91 kDa
Flow Cytometry - Anti-STAT5 (phospho Y694) antibody [Stat5Y694-G11] (ab278764)

Peptide blocking flow cytometric analysis of 3T3 cells secondary antibody only negative control (light blue) or treated with imatinib (red) or pervanadate-treated (green) or imatinib and blocked with phospho-peptide (black) or pervanadate and blocked with phospho peptide (gold) or imatinib and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) using ab278764 at 0.1 µg/mL.
Western blot - Anti-STAT5 (phospho Y694) antibody [Stat5Y694-G11] (ab278764)

Lanes 1-2 : Anti-STAT5 (phospho Y694) antibody [Stat5Y694-G11] (ab278764) at 0.01 µg/ml
Lanes 3-4 : No primary antibody control

Lanes 1 & 3 : K562 cell extract untreated
Lanes 2 & 4 : K562 cell extract treated with imatinib

Predicted band size: 91 kDa
Flow Cytometry - Anti-STAT5 (phospho Y694) antibody [Stat5Y694-G11] (ab278764)

Flow cytometric analysis of 3T3 cells secondary antibody only negative control (blue) or untreated (grey) or treated with pervanadate (orange) using 0.1 µg/mL isotype control or untreated (red) or pervanadate (green) using ab278764 at 0.1 µg/mL.
Anti-STAT5 (phospho Y694) antibody [Stat5Y694-G11] (ab278764)

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