Flow cytometric analysis of K562 cells secondary antibody only negative control (blue) or untreated (grey) or treated with pervanadate (orange) using 0.1 µg/mL isotype control or untreated (red) or pervanadate (green) using ab278764.

All lanes : Anti-STAT5 (phospho Y694) antibody [Stat5Y694-G11] (ab278764) at 0.01 µg/ml

Lane 1 : K562 cell extract untreated
Lane 2 : K562 cell extract treated with imatinib

Predicted band size: 91 kDa

Peptide blocking flow cytometric analysis of 3T3 cells secondary antibody only negative control (light blue) or treated with imatinib (red) or pervanadate-treated (green) or imatinib and blocked with phospho-peptide (black) or pervanadate and blocked with phospho peptide (gold) or imatinib and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) using ab278764 at 0.1 µg/mL.

Lanes 1-2 : Anti-STAT5 (phospho Y694) antibody [Stat5Y694-G11] (ab278764) at 0.01 µg/ml
Lanes 3-4 : No primary antibody control

Lanes 1 & 3 : K562 cell extract untreated
Lanes 2 & 4 : K562 cell extract treated with imatinib

Predicted band size: 91 kDa

Flow cytometric analysis of 3T3 cells secondary antibody only negative control (blue) or untreated (grey) or treated with pervanadate (orange) using 0.1 µg/mL isotype control or untreated (red) or pervanadate (green) using ab278764 at 0.1 µg/mL.