Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody (ab70324)
Key features and details
- Rabbit polyclonal to RNA polymerase II CTD repeat YSPTSPS (phospho S2)
- Suitable for: IHC-P, WB, ELISA
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody
See all RNA polymerase II CTD repeat YSPTSPS primary antibodies -
Description
Rabbit polyclonal to RNA polymerase II CTD repeat YSPTSPS (phospho S2) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ELISAmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Rabbit, Cow, Dog, Pig, Chimpanzee, Drosophila melanogaster, Plants, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
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Immunogen
Synthetic peptide corresponding to Human RNA polymerase II CTD repeat
YSPTSPS
(C terminal) (phospho S2).
Sequence:YSPTSPS
Database link: P24928 -
Positive control
- Whole cell lysate from HeLa cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody (ab70324)All lanes : Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody (ab70324) at 0.02 µg/ml
Lane 1 : Whole cell lysate from HeLa cells with no peptide
Lane 2 : Whole cell lysate from HeLa cells with non-phosphorylated peptide
Lane 3 : Whole cell lysate from HeLa cells with phosphorylated S2 peptide
Lane 4 : Whole cell lysate from HeLa cells with phosphorylated S5 peptide
Lysates/proteins at 50 µg per lane.
Observed band size: 270 kDa why is the actual band size different from the predicted?
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ELISA - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody (ab70324)Serially diluted ab70324 was bound to immobilised phospho- or control peptides (1 microgram per mL). The antibody was detected by HRP-labelled goat anti-rabbit IgG (ab97080; diluted 50000 times) and signal was developed by TMB substrate.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody (ab70324)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right) tissues labelling RNA polymerase II CTD repeat YSPTSPS (phospho S2) with ab70324 at 1/500 (0.4µg/ml). Detection: DAB.
