Anti-CD3 epsilon antibody [OKT-3 (muromonab)] - Chimeric (ab275972)
![Anti-CD3 epsilon antibody [OKT-3 (muromonab)] - Chimeric (ab275972)](https://www.abcam.com/ps/products/275/ab275972/Images/ab275972-411978-anti-CD3epsilon-antibody-ICCIF.jpg "Anti-CD3 epsilon antibody [OKT-3 (muromonab)] - Chimeric (ab275972)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [OKT-3 (muromonab)] to CD3 epsilon - Chimeric
- Suitable for: ICC
- Reacts with: Human
Overview
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Product name
Anti-CD3 epsilon antibody [OKT-3 (muromonab)] - Chimeric
See all CD3 epsilon primary antibodies -
Description
Rabbit monoclonal [OKT-3 (muromonab)] to CD3 epsilon - Chimeric -
Host species
Rabbit -
Specificity
Epitope on the epsilon-subunit within the human CD3 complex.
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Tested Applications & Species
See all applications and species dataApplication Species ICC Human
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Immunogen
Tissue, cells or virus corresponding to Human CD3 epsilon. Human peripheral E+ cells.
Database link: P07766 -
Positive control
- ICC: MOLT-4 cells.
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General notes
This chimeric rabbit antibody was made using the variable domain sequences of the original mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99.98% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
OKT-3 (muromonab) -
Isotype
IgG -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry - Anti-CD3 epsilon antibody [OKT-3 (muromonab)] (ab275972)
Immunofluorescence analysis of paraformaldehyde fixed MOLT-4 (human lymphoblastic leukemia cell line) cells (on Shi-fix coverslips, permeabilized with 0.15% Triton) and labeled OKT-3 with ab275972 at 10 μg/ml for 1h followed by Alexa Fluor®488 secondary antibody (1 μg/ml) (Green). The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom ab275972, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor®488 secondary antibody.
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![Anti-CD3 epsilon antibody [OKT-3 (muromonab)] - Chimeric (ab275972)](https://www.abcam.com/ps/products/275/ab275972/Images/ab275972-2-benefits-of-recombinant-antibodies.png)
Anti-CD3 epsilon antibody [OKT-3 (muromonab)] - Chimeric (ab275972)