Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab108799)
Key features and details
- Sensitivity: 0.32 ng/ml
- Range: 0.391 ng/ml - 25 ng/ml
- Sample type: Cell culture media, Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human alpha 1 Antitrypsin ELISA Kit (SERPINA1)
See all alpha 1 Antitrypsin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5.3% Inter-assay Sample n Mean SD CV% Overall 9.6% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture media, Cerebral Spinal Fluid -
Assay type
Sandwich (quantitative) -
Sensitivity
= 0.32 ng/ml -
Range
0.391 ng/ml - 25 ng/ml -
Recovery
103 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human alpha 1 Antitrypsin ELISA kit (SERPINA1) (ab108799) is a sandwich ELISA kit for the quantitative measurement of alpha 1 Antitrypsin in urine, milk, saliva, CSF, cell lysate and cell culture supernatants.
An alpha 1 antitrypsin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an alpha 1 antitrypsin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of alpha 1 antitrypsin captured in plate.
Get results in 90 minutes with Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab189579) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human alpha 1 Antitrypsin Antibody 1 x 120µl alpha 1 Antitrypsin Microplate (12 x 8 well strips) 1 unit alpha 1 Antitrypsin Standard 1 vial Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml -
Research areas
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Function
Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE). -
Tissue specificity
Ubiquitous. Expressed in leukocytes and plasma. -
Involvement in disease
Alpha-1-antitrypsin deficiency -
Sequence similarities
Belongs to the serpin family. -
Domain
The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable. -
Post-translational
modificationsN-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418. -
Cellular localization
Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix. - Information by UniProt
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Alternative names
- A1A
- A1AT
- A1AT_HUMAN
see all -
Database links
- Entrez Gene: 5265 Human
- Omim: 107400 Human
- SwissProt: P01009 Human
- Unigene: 525557 Human
Images
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ELISA: alpha 1 Antitrypsin (SERPINA1) Human ELISA Kit
Linearity: alpha 1 antitrypsin in urine at different diutions, corrected for the factor of dilution (duplicates +/- SD).
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ELISA: alpha 1 Antitrypsin (SERPINA1) Human ELISA KitLinearity: alpha 1 antitrypsin in human milk at different diutions, corrected for the factor of dilution (duplicates +/- SD)
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ELISA: alpha 1 Antitrypsin (SERPINA1) Human ELISA KitLinearity: alpha 1 antitrypsin in serum and plasma (Li-heparine) at different diutions, corrected for the factor of dilution (duplicates +/- SD)
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ELISA: alpha 1 Antitrypsin (SERPINA1) Human ELISA KitAlpha 1 antitrypsin in human biological fluids (duplicates +/- SD).
The samples were tested in different ranges of dilutions (serum and Li-heparine plasma: 2e3-1e6; CSF: 5e3-5e4; milk: 1e2-1e3; saliva: 2e2-2e3).
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Typical Standard Curve for ab108799 Human alpha 1 Antitrypsin ELISA kitRepresentative Standard Curve using ab108799
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ELISA: alpha 1 Antitrypsin (SERPINA1) Human ELISA KitStandard curve with background signal subtracted (duplicates; +/- SD).
