Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2343] to Integrin alpha 4/CD49D (phospho S988)
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343]
    See all Integrin alpha 4/CD49D primary antibodies

  • Description

    Rabbit monoclonal [EPR2343] to Integrin alpha 4/CD49D (phospho S988)

  • Host species

    Rabbit

  • Specificity

    ab75760 only detects Integrin alpha 4/CD49D phosphorylated on Serine 988 of the mature protein. S988 coresponds to S1021 of the sequence with the 33 aa signal peptide and S1027 of the precursor shown in Figure 4 of PMID: 8436405.

    The antibody detects the mature chain (180 kDa), the precursor (140 kDa) and the cleaved C terminal (70 kDa).

     

  • Tested Applications & Species

    Application Species
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    corresponding to Human Integrin alpha 4/CD49D (phospho S988).
    Database link: P13612

  • Positive control

    • THP1 cell lysates

  • General notes

    This product was previously labelled as Integrin alpha 4.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760)
    Western blot - Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760)
    All lanes : Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760) at 1/1000 dilution (purified)

    Lane 1 : rat spleen lysate
    Lane 2 : mouse spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 150 kDa
    Observed band size: 150,70 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760)
    Western blot - Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760)
    All lanes : Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760) at 1/5000 dilution (purified)

    Lane 1 : untreated Jurkat lysate
    Lane 2 : Jurkat cell lysate treated with Lambda phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 150 kDa
    Observed band size: 140,150,70 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760)
    Western blot - Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760)
    All lanes : Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760) at 1/2000 dilution

    Lane 1 : THP1 cell lysates, untreated
    Lane 2 : THP1 cell lysates, with Lambda
    Phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 150 kDa
    Observed band size: 140,180,70 kDa why is the actual band size different from the predicted?

  • Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760)
    Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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