Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2343] to Integrin alpha 4/CD49D (phospho S988)
Suitable for: WB
Reacts with: Mouse, Rat, Human

Product name

Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343]
See all Integrin alpha 4/CD49D primary antibodies
Description

Rabbit monoclonal [EPR2343] to Integrin alpha 4/CD49D (phospho S988)
Host species

Rabbit
Specificity

ab75760 only detects Integrin alpha 4/CD49D phosphorylated on Serine 988 of the mature protein. S988 coresponds to S1021 of the sequence with the 33 aa signal peptide and S1027 of the precursor shown in Figure 4 of PMID: 8436405.

The antibody detects the mature chain (180 kDa), the precursor (140 kDa) and the cleaved C terminal (70 kDa).
Tested Applications & Species

Application Species
WB
Rat

Human

See all applications and species data
Immunogen

corresponding to Human Integrin alpha 4/CD49D (phospho S988).
Database link: P13612
Positive control
- THP1 cell lysates
General notes
This product was previously labelled as Integrin alpha 4.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
WB	Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR2343
Isotype

IgG
Research areas

Western blot - Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760)

All lanes : Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760) at 1/1000 dilution (purified)

Lane 1 : rat spleen lysate
Lane 2 : mouse spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 150 kDa
Observed band size: 150,70 kDa
why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760)

All lanes : Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760) at 1/5000 dilution (purified)

Lane 1 : untreated Jurkat lysate
Lane 2 : Jurkat cell lysate treated with Lambda phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

Predicted band size: 150 kDa
Observed band size: 140,150,70 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760)

All lanes : Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760) at 1/2000 dilution

Lane 1 : THP1 cell lysates, untreated
Lane 2 : THP1 cell lysates, with Lambda
Phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 150 kDa
Observed band size: 140,180,70 kDa why is the actual band size different from the predicted?
Anti-Integrin alpha 4/CD49D (phospho S988) antibody [EPR2343] (ab75760)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com