Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1355Y] to Integrin alpha 4/CD49D
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Integrin alpha 4/CD49D antibody [EPR1355Y]
    See all Integrin alpha 4/CD49D primary antibodies

  • Description

    Rabbit monoclonal [EPR1355Y] to Integrin alpha 4/CD49D

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Integrin alpha 4/CD49D aa 1000-1100 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Jurkat whole cell lysate (ab7899)

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product was previously labelled as Integrin alpha 4.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280)
    Western blot - Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280)

    Lane 1: Integrin alpha 4 knockout HAP1 cell lysate (20 µg)

    Lane 2: Wild-type HAP1 cell lysate (20 µg)

    Lanes 1 and 2: Merged signal (red and green). Green - ab81280 observed at 150 kDa. Red - loading control, ab8226, observed at 42 kDa.


    ab81280 was shown to recognize Integrin alpha 4 in wild-type HAP1 cells along with additional cross-reactive bands. No bands were observed when Integrin alpha 4 knockout samples were used. Wild-type and Integrin alpha 4 knockout samples were subjected to SDS-PAGE. ab81280 and ab8226 (loading control to beta actin) were diluted 1/2000 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.

  • Western blot - Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280)
    Western blot - Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280)
    All lanes : Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280) at 1/1000 dilution

    All lanes : Jurkat whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 150 kDa
    Observed band size: 150 kDa



    Lanes 1 - 2: Merged signal (red and green). Green - ab81280 observed at 150 kDa. Red - loading control, ab8245, observed at 37 kDa.

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab81280 and ab8245 (loading control) overnight at 4°C. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at a 1:10000 dilution for 1hr at room temperature and then imaged.

  • Western blot - Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280)
    Western blot - Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280)
    Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280) at 1/2000 dilution + Jurkat cell lysate at 10 µg

    Secondary
    HRP labelled goat anti rabbit at 1/2000 dilution

    Predicted band size: 150 kDa
    Observed band size: 150 kDa

  • Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280)
    Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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