Anti-TMS1/ASC antibody [EPR10402(B)] - BSA and Azide free (ab249023)
![Anti-TMS1/ASC antibody [EPR10402(B)] - BSA and Azide free (ab249023)](https://www.abcam.com/ps/products/249/ab249023/Images/ab249023-410317-anti-tms1-asc-antibody-epr10402-b-bsa-and-azide-free-western-blot.jpg "Anti-TMS1/ASC antibody [EPR10402(B)] - BSA and Azide free (ab249023)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10402(B)] to TMS1/ASC - BSA and Azide free
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-TMS1/ASC antibody [EPR10402(B)] - BSA and Azide free
See all TMS1/ASC primary antibodies -
Description
Rabbit monoclonal [EPR10402(B)] to TMS1/ASC - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab249023 is the carrier-free version of ab151700 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab249023 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as TMS1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10402(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TMS1/ASC antibody [EPR10402(B)] - BSA and Azide free (ab249023)All lanes : Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700) at 1/5000 dilution (purified)
Lane 1 : THP-1 (Human monocytic leukemia monocyte) whole cell lysate
Lane 2 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 22 kDaThis data was developed using ab151700, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-TMS1/ASC antibody [EPR10402(B)] - BSA and Azide free (ab249023)](https://www.abcam.com/ps/products/249/ab249023/Images/ab249023-408064-anti-tms1-asc-antibody-epr10402-b-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-TMS1/ASC antibody [EPR10402(B)] - BSA and Azide free (ab249023)This data was developed using ab151700, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: TMS1/ASC knockout HAP1 cell lysate (20 µg)
Lane 3: U937 cell lysate (20 µg)
Lane 4: THP1 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab151700 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
Unpurified ab151700 was shown to specifically react with TMS1/ASC when TMS1/ASC knockout samples were used. Wild-type and TMS1/ASC knockout samples were subjected to SDS-PAGE. Unpurified ab151700 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Immunoprecipitation - Anti-TMS1/ASC antibody [EPR10402(B)] - BSA and Azide free (ab249023)](https://www.abcam.com/ps/products/249/ab249023/Images/ab249023-3-anti-tms1-asc-antibody-epr10402-b-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-TMS1/ASC antibody [EPR10402(B)] - BSA and Azide free (ab249023)This data was developed using ab151700, the same antibody clone in a different buffer formulation.ab151700 (purified) at 1:30 dilution (2ug) immunoprecipitating TMS1/ASC in THP-1 (Human monocytic leukemia monocyte) whole cell lysate.
Lane 1 (input): THP-1 (Human monocytic leukemia monocyte) whole cell lysate 10ug
Lane 2 (+): ab151700 & THP-1 (Human monocytic leukemia monocyte) whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab151700 in THP-1 (Human monocytic leukemia monocyte) whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
![Western blot - Anti-TMS1/ASC antibody [EPR10402(B)] - BSA and Azide free (ab249023)](https://www.abcam.com/ps/products/249/ab249023/Images/ab249023-402294-anti-tms1-asc-antibody-epr10402-b-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-TMS1/ASC antibody [EPR10402(B)] - BSA and Azide free (ab249023)All lanes : Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700) at 1/1000 dilution (unpurified)
Lane 1 : THP1 cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : U937 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 22 kDaThis data was developed using ab151700, the same antibody clone in a different buffer formulation.
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![Flow Cytometry - Anti-TMS1/ASC antibody [EPR10402(B)] - BSA and Azide free (ab249023)](https://www.abcam.com/ps/products/249/ab249023/Images/ab249023-1-anti-tms1-asc-antibody-epr10402-b-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-TMS1/ASC antibody [EPR10402(B)] - BSA and Azide free (ab249023) -
![Anti-TMS1/ASC antibody [EPR10402(B)] - BSA and Azide free (ab249023)](https://www.abcam.com/ps/products/249/ab249023/Images/ab249023-6-benefits-of-recombinant-antibodies.png)
Anti-TMS1/ASC antibody [EPR10402(B)] - BSA and Azide free (ab249023)
