Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Catalase antibody [EPR1928Y] - BSA and Azide free (ab247440)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1928Y] to Catalase - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Catalase antibody [EPR1928Y] - BSA and Azide free
    See all Catalase primary antibodies

  • Description

    Rabbit monoclonal [EPR1928Y] to Catalase - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247440 is the carrier-free version of ab76110. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247440 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Catalase antibody [EPR1928Y] - BSA and Azide free (ab247440)
    Western blot - Anti-Catalase antibody [EPR1928Y] - BSA and Azide free (ab247440)

    This data was developed using ab76110, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: Catalase knockout HAP1 cell lysate (20 µg) 

    Lane 3: HeLa cell lysate (20 µg)

    Lane 4: Jurkat cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab76110 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab76110 was shown to specifically react with Catalase when Catalase knockout samples were used. Wild-type and Catalase knockout samples were subjected to SDS-PAGE. ab76110 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EPR1928Y] - BSA and Azide free (ab247440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EPR1928Y] - BSA and Azide free (ab247440)
    This data was developed using ab76110, the same antibody clone in a different buffer formulation.ab76110 at 1/100-1/250 dilution staining Catalase in human liver by Immunohistochemistry, Paraffin-embedded tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Western blot - Anti-Catalase antibody [EPR1928Y] - BSA and Azide free (ab247440)
    Western blot - Anti-Catalase antibody [EPR1928Y] - BSA and Azide free (ab247440)
    All lanes : Anti-Catalase antibody [EPR1928Y] (ab76110) at 1/1000 dilution

    Lane 1 : TF cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 60 kDa
    Observed band size: 60 kDa



    This data was developed using ab76110, the same antibody clone in a different buffer formulation.

     

  • Anti-Catalase antibody [EPR1928Y] - BSA and Azide free (ab247440)
    Anti-Catalase antibody [EPR1928Y] - BSA and Azide free (ab247440)

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