Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Thursday March 04, 2021

Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8416(2)] (ab191431)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR8416(2)] to ACAT2/Acetyl-CoA acetyltransferase
Suitable for: WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8416(2)]
See all ACAT2/Acetyl-CoA acetyltransferase primary antibodies
Description

Rabbit monoclonal [EPR8416(2)] to ACAT2/Acetyl-CoA acetyltransferase
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Rat

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HepG2, HeLa, Jurkat, K562, PC12 and NIH 3T3 cell lysates.
General notes
This product was previously labelled as ACAT2, Acetyl-CoA acetyltransferase

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR8416(2)
Isotype

IgG
Research areas

Western blot - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8416(2)] (ab191431)

All lanes : Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8416(2)] (ab191431) at 1/10000 dilution

Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K562 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 41 kDa
Immunoprecipitation - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8416(2)] (ab191431)

Western blot analysis of immunoprecipitation pellet from HepG2 cell lysate immunoprecipitated using ab191431 at 1/20 dilution (lane 1) or PBS control (lane 2).
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Western blot - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8416(2)] (ab191431)

All lanes : Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8416(2)] (ab191431) at 1/1000 dilution

Lane 1 : PC12 cell lysate
Lane 2 : NIH 3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 41 kDa
Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8416(2)] (ab191431)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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