Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (ab131215)
![Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (ab131215)](https://www.abcam.com/ps/products/131/ab131215/Images/ab131215-145517-anti-acetyl-coa-acetyltransferase-antibody-epr8417-western-blot.jpg "Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (ab131215)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8417] to ACAT2/Acetyl-CoA acetyltransferase
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417]
See all ACAT2/Acetyl-CoA acetyltransferase primary antibodies -
Description
Rabbit monoclonal [EPR8417] to ACAT2/Acetyl-CoA acetyltransferase -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human ACAT2/Acetyl-CoA acetyltransferase aa 200-300. The exact sequence is proprietary.
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Positive control
- Human liver tissue; HepG2, HeLa, Jurkat and K562 cell lysates.
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General notes
This product was previously labelled as ACAT2, Acetyl-CoA acetyltransferase
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.91 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8417 -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipoprotein metabolism
Images
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Western blot - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (ab131215)All lanes : Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (ab131215) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 41 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (ab131215)](https://www.abcam.com/ps/products/131/ab131215/Images/ab131215-145516-anti-acetyl-coa-acetyltransferase-antibody-epr8417-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (ab131215)Immunohistochemical analysis of paraffin embedded Human liver tissue labelling ACAT2/Acetyl-CoA acetyltransferase with ab131215 antibody at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (ab131215)](https://www.abcam.com/ps/products/131/ab131215/Images/ab131215-145515-anti-acetyl-coa-acetyltransferase-antibody-epr8417.jpg)
OI-RD Scanning - Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (ab131215) -
![Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (ab131215)](https://www.abcam.com/ps/products/131/ab131215/Images/ab131215-4-benefits-of-recombinant-antibodies.png)
Anti-ACAT2/Acetyl-CoA acetyltransferase antibody [EPR8417] (ab131215)
