Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Fas antibody [EPR5700] - BSA and Azide free (ab178076)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5700] to Fas - BSA and Azide free
  • Suitable for: ICC, WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Fas antibody [EPR5700] - BSA and Azide free
    See all Fas primary antibodies

  • Description

    Rabbit monoclonal [EPR5700] to Fas - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to residues in Human CD95 (UniProt ID: P25445).

  • Positive control

    • WB: HeLa cell lysate. IHC-P: Human tonsil tissue. ICC: Raji cells

  • General notes

    Ab178076 is the carrier-free version of ab133619. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab178076 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Fas antibody [EPR5700] - BSA and Azide free (ab178076)
    Western blot - Anti-Fas antibody [EPR5700] - BSA and Azide free (ab178076)
    All lanes : Anti-Fas antibody [EPR5700] (ab133619) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : FAS knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 37 kDa
    Observed band size: 37 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab133619).

    Lanes 1-2: Merged signal (red and green). Green - ab133619 observed at 37 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab133619 Anti-Fas antibody [EPR5700] was shown to specifically react with Fas in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265260 (knockout cell lysate ab256911) was used. Wild-type and Fas knockout samples were subjected to SDS-PAGE. ab133619 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fas antibody [EPR5700] - BSA and Azide free (ab178076)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fas antibody [EPR5700] - BSA and Azide free (ab178076)

    Anti-Fas antibody [EPR5700] (ab133619)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling Fas with ab133619 at a dilution of 1:500. Heat mediated antigen retrieval was performed using AR9 antigen retrieval solution, and microwave treatment for 15 min at 20% power. Anti-Rabbit/Mouse HRP polymer (PerkinElmer Opal Polymer HRP Ms Plus Rb) was used as secondary antibody. Opal tyramide amplification was performed using Opal 520 fluorophore. Counterstained with DAPI stain. Image scanned with Vectra 3.0 and analyzed via Phenochart software.
    This image was courteously provided by Dr. Houssein Abdul Sater, Georgia Cancer Center.

  • Immunocytochemistry - Anti-Fas antibody [EPR5700] - BSA and Azide free (ab178076)
    Immunocytochemistry - Anti-Fas antibody [EPR5700] - BSA and Azide free (ab178076)
    This data was developed using the same antibody clone in a different buffer formulation (ab133619).
    Immunocytochemistry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) labeling Fas with purified ab133619 at 1/50 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.31 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
    Negative control: PBS instead of the primary antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fas antibody [EPR5700] - BSA and Azide free (ab178076)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fas antibody [EPR5700] - BSA and Azide free (ab178076)

    Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelling CD95 with ab133619 antibody at a dilution of 1/250.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133619).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunocytochemistry - Anti-Fas antibody [EPR5700] - BSA and Azide free (ab178076)
    Immunocytochemistry - Anti-Fas antibody [EPR5700] - BSA and Azide free (ab178076)

    Clone EPR5700 (ab178076) has been successfully conjugated by Abcam. This image was generated using Anti-Fas antibody [EPR5700] (Alexa Fluor® 488). Please refer to ab204670 for protocol details.

    ab204670 staining Fas in Raji cells. The cells were fixed with 80% methanol (5 min) and then incubated in 1%BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated overnight at +4°C with ab204670 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in Raji cells fixed with 4% formaldehyde (10 min).

  • Immunocytochemistry - Anti-Fas antibody [EPR5700] - BSA and Azide free (ab178076)
    Immunocytochemistry - Anti-Fas antibody [EPR5700] - BSA and Azide free (ab178076)

    Clone EPR5700 (ab178076) has been successfully conjugated by Abcam. This image was generated using Anti-Fas antibody [EPR5700] (Alexa Fluor® 647). Please refer to ab204671 for protocol details.

    ab204671 staining Fas in Raji cells. The cells were fixed with 4% formaldehyde (10 min) and then incubated in 1%BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated overnight at +4°C with ab204671 at 1/50 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

     

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Anti-Fas antibody [EPR5700] - BSA and Azide free (ab178076)
    Anti-Fas antibody [EPR5700] - BSA and Azide free (ab178076)

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