Anti-HSF1 (phospho S320) antibody [EPR1712] (ab76183)
![Anti-HSF1 (phospho S320) antibody [EPR1712] (ab76183)](https://www.abcam.com/ps/products/76/ab76183/Images/ab76183-91541-anti-hsf1-phospho-s320-antibody-epr1712-immunohistochemistry.jpg "Anti-HSF1 (phospho S320) antibody [EPR1712] (ab76183)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1712] to HSF1 (phospho S320)
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-HSF1 (phospho S320) antibody [EPR1712]
See all HSF1 primary antibodies -
Description
Rabbit monoclonal [EPR1712] to HSF1 (phospho S320) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
A synthetic phospho-peptide from residues surrounding serine 320 of human HSF1.
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Positive control
- HeLa whole cell lysate (ab150035), human muscle tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1712 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSF1 (phospho S320) antibody [EPR1712] (ab76183)
Immunohistochemical analysis of paraffin-embedded human muscles using ab76183 at 1/100 dilution.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Western blot - Anti-HSF1 (phospho S320) antibody [EPR1712] (ab76183)](https://www.abcam.com/ps/products/76/ab76183/Images/ab76183-91540-anti-hsf1-phospho-s320-antibody-epr1712-western-blot.jpg)
Western blot - Anti-HSF1 (phospho S320) antibody [EPR1712] (ab76183)All lanes : Anti-HSF1 (phospho S320) antibody [EPR1712] (ab76183) at 1/20000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate, heat treated at 42°C
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit-HRP at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 57 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
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![Anti-HSF1 (phospho S320) antibody [EPR1712] (ab76183)](https://www.abcam.com/ps/products/76/ab76183/Images/ab76183-3-benefits-of-recombinant-antibodies.png)
Anti-HSF1 (phospho S320) antibody [EPR1712] (ab76183)
