Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] (ab81281)
![Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] (ab81281)](https://www.abcam.com/ps/products/81/ab81281/Images/ab81281-96465-anti-hsf1-phospho-s303-s307-antibody-ep1711y-western-blot.jpg "Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] (ab81281)")
Key features and details
- Rabbit monoclonal [EP1711Y] to HSF1 (phospho S303 + S307)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y]
See all HSF1 primary antibodies -
Description
Rabbit monoclonal [EP1711Y] to HSF1 (phospho S303 + S307) -
Host species
Rabbit -
Specificity
ab81281 antibody recognises HSF1 (phospho S303 + S307) -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human HSF1 aa 250-350. The exact sequence is proprietary.
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Positive control
- HeLa cell lysates, Human breast carcinoma.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1711Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] (ab81281)All lanes : Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] (ab81281) at 1/50000 dilution
Lane 1 : HeLa cell lysate untreated
Lane 2 : HeLa cell lysate AP treated
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRp labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 57 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] (ab81281)](https://www.abcam.com/ps/products/81/ab81281/Images/ab81281-96464-anti-hsf1-phospho-s303-s307-antibody-ep1711y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] (ab81281)Immunohistochemical analysis of paraffin-embedded human breast carcinoma using 1/100 ab81281
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
