Anti-HSF1 (phospho S303) antibody (ab47369)
Key features and details
- Rabbit polyclonal to HSF1 (phospho S303)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HSF1 (phospho S303) antibody
See all HSF1 primary antibodies -
Description
Rabbit polyclonal to HSF1 (phospho S303) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthesized phosphopeptide derived from HSF1 around the phosphorylation site of serine 303 (Human).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HSF1 (phospho S303) antibody (ab47369)Lanes 1-2 : HSF1 antibody at 1/500 dilution
Lanes 3-4 : Anti-HSF1 (phospho S303) antibody (ab47369) at 1/500 dilution
Lanes 1 & 4 : Extracts from MCF cells treated with TNF alpha (20ng/ml, 30min). No peptide.
Lane 2 : Extracts from MCF cells treated with TNF alpha (20ng/ml, 30min). Synthetic phosphopeptide present.
Lane 3 : Extracts from MCF cells not treated
with TNF alpha. No peptide.
Predicted band size: 57 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSF1 (phospho S303) antibody (ab47369)ab47369 (1/50) staining HSF1 in paraffin-embedded human breast carcinoma tissue. Right-hand panel corresponds to a negative control for which ab47369 was pre-incubated with the immunising (blocking) peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-HSF1 (phospho S303) antibody (ab47369)ICC/IF image of ab47369 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47369, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
