Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD23 antibody [EPR3617] (ab92495)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3617] to CD23
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CD23 antibody [EPR3617]
    See all CD23 primary antibodies

  • Description

    Rabbit monoclonal [EPR3617] to CD23

  • Host species

    Rabbit

  • Specificity

    Recognizes both membrane bound and soluble forms of CD23.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human CD23 aa 300-400 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Human lymph and tonsil lysates; IHC-P: Human tonsil tissue; Flow Cyt: Human peripheral blood mononuclear cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-CD23 antibody [EPR3617] (ab92495)
    Western blot - Anti-CD23 antibody [EPR3617] (ab92495)
    Anti-CD23 antibody [EPR3617] (ab92495) at 1/1000 dilution (Purified) + Human lymph lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 36 kDa
    Observed band size: 37,45 kDa
    why is the actual band size different from the predicted?



    The bands detected by ab92495 are consistent with what have been described in PMID: 20876574 and PMID: 17389606.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling CD23 with Purified ab92495 at 1/400 dilution (0.5 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Flow Cytometry - Anti-CD23 antibody [EPR3617] (ab92495)
    Flow Cytometry - Anti-CD23 antibody [EPR3617] (ab92495)

    Flow Cytometry analysis of Human peripheral blood mononuclear (PBMC) cells labeling CD23 with purified ab92495 at 1:20 dilution (10 µg/ml) (Red). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Left).

    Cells were stained with rabbit IgG (Left) or ab92495 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor® 647. Gated on viable cells.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)

    ab92495 (unpurified), at a 1/100 dilution, staining CD23 in formalin fixed, paraffin embedded Human tonsil tissue by Immunohistochemistry. Detection: DAB staining.

    Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)

    ab92495 (unpurified) showing positive staining in Normal tonsil tissue.

    Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)

    ab92495 (unpurified) showing negative staining in Normal heart tissue.

    Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)

    ab92495 (unpurified) showing negative staining in Normal breast tissue.

    Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [EPR3617] (ab92495)

    ab92495 (unpurified) showing negative staining in Normal brain tissue.

    Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-CD23 antibody [EPR3617] (ab92495)
    OI-RD Scanning - Anti-CD23 antibody [EPR3617] (ab92495)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-CD23 antibody [EPR3617] (ab92495)
    Anti-CD23 antibody [EPR3617] (ab92495)

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