Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD23 antibody [SP163] (ab135386)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP163] to CD23
  • Suitable for: ICC/IF, Flow Cyt, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CD23 antibody [SP163]
    See all CD23 primary antibodies

  • Description

    Rabbit monoclonal [SP163] to CD23

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human CD23 aa 1-250. The exact sequence is proprietary.
    Database link: P06734

  • Positive control

    • Flow Cyt: Raji cells. WB: Raji cell lysate. IHC-P: Human tonsil tissue. ICC/IF: Raji cells

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-CD23 antibody [SP163] (ab135386)
    Immunocytochemistry/ Immunofluorescence - Anti-CD23 antibody [SP163] (ab135386)

    Immunocytochemistry/ Immunofluorescence analysis of Raji (human Burkitt's lymphoma B lymphocyte) cells labeling CD23 with purified ab135386 (7.48 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-CD23 antibody [SP163] (ab135386)
    Flow Cytometry - Anti-CD23 antibody [SP163] (ab135386)

    Flow cytometric analysis of rabbit anti-CD23 (SP163) antibody ab135386 (1/100) in Raji (Human Burkitt's lymphoma cell line) cells (green) compared to negative control of rabbit IgG (blue).

  • Western blot - Anti-CD23 antibody [SP163] (ab135386)
    Western blot - Anti-CD23 antibody [SP163] (ab135386)
    Anti-CD23 antibody [SP163] (ab135386) at 1/25 dilution + Raji (Human Burkitt's lymphoma cell line) cell lysate

    Predicted band size: 36 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP163] (ab135386)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP163] (ab135386)

    Immunohistochemical analysis of paraffin embedded human tonsil tissue labeling CD23 with ab135386 antibody at a dilution of 1/50.

  • Anti-CD23 antibody [SP163] (ab135386)
    Anti-CD23 antibody [SP163] (ab135386)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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