Astana Biomed Group, an authorized Abcam distributor in Central Asia

PE Anti-LAG-3 antibody [EPR20261] (ab225484)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • PE Rabbit monoclonal [EPR20261] to LAG-3
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm

Overview

  • Product name

    PE Anti-LAG-3 antibody [EPR20261]
    See all LAG-3 primary antibodies

  • Description

    PE Rabbit monoclonal [EPR20261] to LAG-3

  • Host species

    Rabbit

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human LAG-3 aa 1-300. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P18627

  • Positive control

    • Flow Cyt: A431 cells and HEK-293T cells transfected with a GFP-tagged human LAG3 construct.

  • General notes

    This product was previously labelled as Lymphocyte Activation Gene 3.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - PE Anti-LAG-3 antibody [EPR20261] (ab225484)
    Flow Cytometry - PE Anti-LAG-3 antibody [EPR20261] (ab225484)

    Flow cytometric analysis of HEK-293T (Human epithelial cell line from embryonic kidney) cells transfected with a GFP-tagged human LAG3 construct labelling LAG-3 with ab225484 at 1/50 dilution.

    Note: Fresh cells without fixation and permeabilization were used to perform FC testing. Only GFP positive population results in LAG3 positive staining (Q2).

  • Flow Cytometry - PE Anti-LAG-3 antibody [EPR20261] (ab225484)
    Flow Cytometry - PE Anti-LAG-3 antibody [EPR20261] (ab225484)

    Overlay histogram showing A431 cells stained with ab225284 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225484, 1/1000 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

  • PE Anti-LAG-3 antibody [EPR20261] (ab225484)
    PE Anti-LAG-3 antibody [EPR20261] (ab225484)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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