Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)
![Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)](https://www.abcam.com/ps/products/76/ab76076/Images/ab76076-351099-anti-hsf1-phospho-s326-antibody-ep1713y-westernblot-human.jpg "Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1713Y] to HSF1 (phospho S326)
- Suitable for: Dot blot, WB
- Reacts with: Human
Overview
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Product name
Anti-HSF1 (phospho S326) antibody [EP1713Y]
See all HSF1 primary antibodies -
Description
Rabbit monoclonal [EP1713Y] to HSF1 (phospho S326) -
Host species
Rabbit -
Specificity
ab76076 detects HSF1 phosphorylated on Ser326. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
corresponding to Human HSF1 aa 300-400.
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Positive control
- WB: HeLa cell lysates and HeLa cell lysate treated with heat (44ºC)
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1713Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)All lanes : Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076) at 0.169 µg/ml
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa treated with heat at 43? for 30 minutes whole cell lysate
Lane 3 : HeLa treated with heat at 43? for 30 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase.
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.02 µg/ml
Predicted band size: 57 kDa
Observed band size: 82 kDa why is the actual band size different from the predicted?Blocking/Diluting Buffer and concentration: 5% NFDM /TBST
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![Western blot - Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)](https://www.abcam.com/ps/products/76/ab76076/Images/ab76076-344755-Anti-hsf1-phosphoS326-antibody-EP1713Y-westernblot-human.jpg)
Western blot - Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)All lanes : Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076) at 1/10000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with heat (42 ?) whole cell lysates
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with heat (42 ?) whole cell lysates. Then the membrane was incubated with phosphatase
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 82 kDa why is the actual band size different from the predicted?
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![Dot Blot - Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)](https://www.abcam.com/ps/products/76/ab76076/Images/ab76076-6-anti-hsf1-phospho-s326-antibody-dotblot-peptides-human.jpg)
Dot Blot - Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)Dot blot analysis of HSF1 (phospho S326) peptide (Lane 1), HSF1 non-phospho peptide (Lane 2) labelling HSF1 (phospho S326) with purified ab76076 at 0.338 ug/mL. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at 0.01 ug/mL.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)](https://www.abcam.com/ps/products/76/ab76076/Images/ab76076-8-benefits-of-recombinant-antibodies.png)
Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)
