Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HSF1 (phospho S326) antibody [EP1713Y] - BSA and Azide free (ab240928)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1713Y] to HSF1 (phospho S326) - BSA and Azide free
  • Suitable for: Dot blot, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-HSF1 (phospho S326) antibody [EP1713Y] - BSA and Azide free
    See all HSF1 primary antibodies

  • Description

    Rabbit monoclonal [EP1713Y] to HSF1 (phospho S326) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody detects HSF1 phosphorylated on Ser326.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa cell lysates and HeLa cell lysate treated with heat (44ºC)

  • General notes

    ab240928 is the carrier-free version of ab76076 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab240928 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-HSF1 (phospho S326) antibody [EP1713Y] - BSA and Azide free (ab240928)
    Western blot - Anti-HSF1 (phospho S326) antibody [EP1713Y] - BSA and Azide free (ab240928)
    All lanes : Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076) at 1/10000 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with heat (42 ?) whole cell lysates
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with heat (42 ?) whole cell lysates. Then the membrane was incubated with phosphatase

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 57 kDa
    Observed band size: 82 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76076).

  • Western blot - Anti-HSF1 (phospho S326) antibody [EP1713Y] - BSA and Azide free (ab240928)
    Western blot - Anti-HSF1 (phospho S326) antibody [EP1713Y] - BSA and Azide free (ab240928)
    All lanes : Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076) at 0.169 µg/ml

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HeLa treated with heat at 43? for 30 minutes whole cell lysate
    Lane 3 : HeLa treated with heat at 43? for 30 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.02 µg/ml

    Predicted band size: 57 kDa
    Observed band size: 82 kDa why is the actual band size different from the predicted?



    Blocking/Diluting Buffer and concentration: 5% NFDM /TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76076).

  • Dot Blot - Anti-HSF1 (phospho S326) antibody [EP1713Y] - BSA and Azide free (ab240928)
    Dot Blot - Anti-HSF1 (phospho S326) antibody [EP1713Y] - BSA and Azide free (ab240928)

    Dot blot analysis of HSF1 (phospho S326) peptide (Lane 1), HSF1 non-phospho peptide (Lane 2) labelling HSF1 (phospho S326) with purified ab76076 at 0.338 ug/mL. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at 0.01 ug/mL.

    Blocking and dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76076).

  • Anti-HSF1 (phospho S326) antibody [EP1713Y] - BSA and Azide free (ab240928)
    Anti-HSF1 (phospho S326) antibody [EP1713Y] - BSA and Azide free (ab240928)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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