Mouse ICAM2 ELISA Kit (CD102) (ab205572)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 4 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse ICAM2 ELISA Kit (CD102)
See all ICAM2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 8 3.16% Inter-assay Sample n Mean SD CV% Overall 3 2.09% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
4 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 98 96% - 99% Cell culture media 90 88% - 93% Hep Plasma 103 101% - 105% EDTA Plasma 103 100% - 108% Cit plasma 108 107% - 111% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Goat, Cow, Pig -
Product overview
Mouse ICAM2 ELISA Kit (CD102) (ab205572) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ICAM2 (CD102) protein in cell culture supernatant, serum, and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse ICAM2 (CD102) with 4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM2 is an integral membrane protein that has 2 immunoglobulin-like domains, and may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse ICAM2 Capture Antibody 1 x 600µl 10X Mouse ICAM2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse ICAM2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
ICAM2 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin like C2 type domains, and bind to the leukocyte adhesion LFA1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA1 dependent cell adhesion. It mediates adhesive interactions important for antigen specific immune response, NK cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. -
Cellular localization
Type 1 membrane protein -
Alternative names
- CD102
- ICAM 2
- Intercellular adhesion molecule 2
- Ly60
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Database links
- Entrez Gene: 15896 Mouse
- SwissProt: P35330 Mouse
Images
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Other - Mouse ICAM2 ELISA Kit (CD102) (ab205572)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of ICAM2 standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Titration of mouse serum, citrate plasma, heparin plasma, and EDTA plasma within the working range of the assay.The 1X dilution was 3.13% for the mouse serum, 50.0% for plasma citrate and EDTA, and 25% for plasma heparin. Interpolated data values (mean +/- SD, n = 2) are graphed.
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Titration of stimulated lung (5 µg/mL LPS (+)), unstimulated lung (-), and unstimulated heart (-) supernatant.The 1X dilution was 25.0% for the lung supernatants and 6.25% for the heart supernatant. Interpolated data values (mean +/- SD, n = 2) are graphed.
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Sandwich ELISA - Mouse ICAM2 ELISA Kit (CD102) (ab205572)To learn more about the advantages of recombinant antibodies see here.
