Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Lumican antibody [EPR22511-63] (ab252925)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22511-63] to Lumican
Suitable for: WB, IHC-P, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-Lumican antibody [EPR22511-63]
See all Lumican primary antibodies
Description

Rabbit monoclonal [EPR22511-63] to Lumican
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
IP	Mouse Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human colon,skin, kidney and placenta tissue lysates;Saos-2 whole cell lysate; mose skin tissue lysate; rat skin tissue lysate. IP: Mouse skin lysate; human skin lysate. IHC-P: Human colon tissue; mouse skin tissue; rat colon tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22511-63
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR22511-63] (ab252925)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Lumican with ab252925 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in stroma cells of human colon (PMID:17089042;
PMID:23399832) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-Lumican antibody [EPR22511-63] (ab252925)

All lanes : Anti-Lumican antibody [EPR22511-63] (ab252925) at 1/1000 dilution

Lane 1 : Human colon tissue lysate
Lane 2 : Human skin tissue lysate
Lane 3 : Human placenta tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 38 kDa
Observed band size: 50,70 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times.

Lane 1: 48 seconds; Lane 2: 7.75 seconds; Lane 3: 37 seconds.

The molecular weight observed is consistent with what has been described in the literature (PMID:28459201,22266188).
Immunoprecipitation - Anti-Lumican antibody [EPR22511-63] (ab252925)

Lumican was immunoprecipitated from 0.35 mg of human skin tissue lysate with ab252925 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab252925 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

Lane 1: Human skin tissue lysate 10 μg (Input).
Lane 2: ab252925 IP in human skin tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252925 in human skin tissue lysate lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR22511-63] (ab252925)

Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling Lumican with ab252925 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in stroma cells of mouse skin (PMID:17089042) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-Lumican antibody [EPR22511-63] (ab252925)

All lanes : Anti-Lumican antibody [EPR22511-63] (ab252925) at 1/1000 dilution

Lane 1 : Human kidney tissue lysate
Lane 2 : Saos-2 (human osteosarcoma cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 38 kDa
Observed band size: 50,70 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID:28459201,22266188).

This blot was developed using a higher sensitivity ECL substrate.
Western blot - Anti-Lumican antibody [EPR22511-63] (ab252925)

All lanes : Anti-Lumican antibody [EPR22511-63] (ab252925) at 1/1000 dilution

Lane 1 : Mouse skin tissue lysate
Lane 2 : Rat skin tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 38 kDa

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times.

Lane 1: 15 seconds; Lane 2: 7.75 seconds.

The molecular weight observed is consistent with what has been described in the literature (PMID:22266188).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR22511-63] (ab252925)

Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Lumican with ab252925 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in stroma cells of rat colon (PMID:17089042;
PMID:23399832) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunoprecipitation - Anti-Lumican antibody [EPR22511-63] (ab252925)

Lumican was immunoprecipitated from 0.35 mg of mouse skin tissue lysate with ab252925 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab252925 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

Lane 1: Mouse skin tissue lysate 10 μg (Input).
Lane 2: ab252925 IP in mouse skin tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252925 in mouse skin tissue lysate lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
Anti-Lumican antibody [EPR22511-63] (ab252925)

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