Anti-Casein Kinase 2 beta antibody [EPR1994] (ab133576)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1994] to Casein Kinase 2 beta
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-Casein Kinase 2 beta antibody [EPR1994]
See all Casein Kinase 2 beta primary antibodies -
Description
Rabbit monoclonal [EPR1994] to Casein Kinase 2 beta -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide corresponding to a region within Human Casein Kinase 2 beta (UniProt P67870).
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Positive control
- Human breast tissue; HepG2, HeLa, SH SY5Y and HUVEC cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.94 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1994 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Casein Kinase 2 beta antibody [EPR1994] (ab133576) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : SH SY5Y cell lysate
Lane 4 : HUVEC cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 24 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
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Immunohistochemical analysis of Casein Kinase 2 beta in formalin fixed, paraffin embedded Human breast tissue, using ab133576 at a 1/50 dilution.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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