Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Casein Kinase 2 beta antibody [EPR1994] (ab133576)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR1994] to Casein Kinase 2 beta
Suitable for: IHC-P, WB
Reacts with: Human

Product name

Anti-Casein Kinase 2 beta antibody [EPR1994]
See all Casein Kinase 2 beta primary antibodies
Description

Rabbit monoclonal [EPR1994] to Casein Kinase 2 beta
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to a region within Human Casein Kinase 2 beta (UniProt P67870).
Positive control
- Human breast tissue; HepG2, HeLa, SH SY5Y and HUVEC cell lysates
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 1.94 x 10 ^-10 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR1994
Isotype

IgG
Research areas

Western blot - Anti-Casein Kinase 2 beta antibody [EPR1994] (ab133576)

All lanes : Anti-Casein Kinase 2 beta antibody [EPR1994] (ab133576) at 1/1000 dilution

Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : SH SY5Y cell lysate
Lane 4 : HUVEC cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 24 kDa
Observed band size: 25 kDa
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody [EPR1994] (ab133576)

Immunohistochemical analysis of Casein Kinase 2 beta in formalin fixed, paraffin embedded Human breast tissue, using ab133576 at a 1/50 dilution.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
OI-RD Scanning - Anti-Casein Kinase 2 beta antibody [EPR1994] (ab133576)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-Casein Kinase 2 beta antibody [EPR1994] (ab133576)

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