Anti-Casein Kinase 2 beta (phospho S209) antibody (ab12861)
Key features and details
- Rabbit polyclonal to Casein Kinase 2 beta (phospho S209)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Casein Kinase 2 beta (phospho S209) antibody
See all Casein Kinase 2 beta primary antibodies -
Description
Rabbit polyclonal to Casein Kinase 2 beta (phospho S209) -
Host species
Rabbit -
Specificity
Does not cross react with Casein Kinase 2 alpha [pS360] (mouse), [pS362], [pS370], or Casein Kinase 2 beta pS2]. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
A synthetic phosphopeptide (Human) from the region of casein kinase 2 that contains serine 209.
-
Positive control
- HeLa cell lysates or K562 cell lysates.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Casein Kinase 2 beta. The final product is generated by affinity chromatography using a Casein Kinase 2 beta derived peptide that is phosphorylated at serine 209. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Casein Kinase 2 beta (phospho S209) antibody (ab12861)
Peptide Competition and Phosphatase Treatment
Lysates prepared from HeLa cells were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda phosphatase (5), blocked with a 3% Milk-TBST buffer for one hour at room temperature, and incubated with ab12861 antibody for two hours at room temperature in a 3% Milk-TBST buffer, following prior incubation with: no peptide (1, 5), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphoserine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal method.The data show that only the peptide corresponding to ab12861 blocks the signal, verifying the specificity of the antibody.
Peptide Competition and Phosphatase Treatment
