Anti-PLGF antibody (ab83906)
Key features and details
- Rabbit polyclonal to PLGF
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PLGF antibody
See all PLGF primary antibodies -
Description
Rabbit polyclonal to PLGF -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide corresponding to Human PLGF aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab95428) -
Positive control
- Recombinant Human PLGF1 protein (ab50127) can be used as a positive control in WB. This antibody gave a positive signal in Human Placenta Tissue Lysate, and JEG-3, SW480 and RAW 264.7 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab83906 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseHumanAll applications CowPigApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 26 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 26 kDa).Target
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Function
Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. -
Tissue specificity
While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas. -
Sequence similarities
Belongs to the PDGF/VEGF growth factor family. -
Domain
Isoform PlGF-2 contains a basic insert which acts as a cell retention signal. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Secreted. The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin. - Information by UniProt
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Database links
- Entrez Gene: 5228 Human
- Entrez Gene: 18654 Mouse
- Omim: 601121 Human
- SwissProt: P49763 Human
- SwissProt: P49764 Mouse
- Unigene: 252820 Human
- Unigene: 4809 Mouse
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Alternative names
- D12S1900 antibody
- Pgf antibody
- PGFL antibody
see all
Images
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Western blot - Anti-PLGF antibody (ab83906)All lanes : Anti-PLGF antibody (ab83906) at 1 µg/ml
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes
Ab83906 detects a band of approximately 55-kDa. While this differs to its predicted molecular weight of 26-kDa, this migration has been observed in the literature (PMID: 10403932).
Datasheets and documents
References (0)
ab83906 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-PLGF antibody (ab83906)All lanes : Anti-PLGF antibody (ab83906) at 1 µg/ml
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes
Ab83906 detects a band of approximately 55-kDa. While this differs to its predicted molecular weight of 26-kDa, this migration has been observed in the literature (PMID: 10403932).
