Anti-PLGF antibody - N-terminal (ab9542)
Key features and details
- Rabbit polyclonal to PLGF - N-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
-
Product name
Anti-PLGF antibody - N-terminal
See all PLGF primary antibodies -
Description
Rabbit polyclonal to PLGF - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Recombinant fragment
-
Immunogen
Synthetic peptide corresponding to Human PLGF (N terminal). Highly purified N-terminal 20 aa synthetic peptide (Human).
Database link: P49763 -
General notes
According to Swissprot P49763; PLGF exists in three isoforms PLGF 1, 2 and 3. The N-terminal immunogen sequence of ab9542 is present in all isoforms so extra bands might be observed near the band of interest.
Properties
-
Form
Lyophilized:Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-PLGF antibody - N-terminal (ab9542)
Western blot of chromatography purified, recombinant PLGF-1/-2 and VEGF165 immobilized to PVDF-membrane.
PlGF antibody shows no cross-reactivity to VEGF165. -
Immunocytochemistry/ Immunofluorescence - Anti-PLGF antibody - N-terminal (ab9542)ICC/IF image of ab9542 stained HepG2 (human liver hepatocellular carcinoma cell line) cells. The cells were fixed in 4% formaldehyde for 10 minutes and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9542, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
