Anti-TCF3 / E2A antibody - N-terminal (ab229605)
Key features and details
- Rabbit polyclonal to TCF3 / E2A - N-terminal
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TCF3 / E2A antibody - N-terminal
See all TCF3 / E2A primary antibodies -
Description
Rabbit polyclonal to TCF3 / E2A - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human TCF3/ E2A (N terminal). The exact sequence is proprietary.
Database link: P15923 -
Positive control
- WB: Jurkat, Raji, and NCI-H929 whole cell extracts; NCI-H929 nuclear extract. IP: Jurkat whole cell extract. ICC/IF: MCF7 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TCF3 / E2A antibody - N-terminal (ab229605)All lanes : Anti-TCF3 / E2A antibody - N-terminal (ab229605) at 1/2500 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extract
Lane 3 : NCI-H929 whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 68 kDa7.5% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-TCF3 / E2A antibody - N-terminal (ab229605)4% paraformaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for TCF3 / E2A (green) using ab229605 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
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Western blot - Anti-TCF3 / E2A antibody - N-terminal (ab229605)All lanes : Anti-TCF3 / E2A antibody - N-terminal (ab229605) at 1/3000 dilution
Lane 1 : NCI-H929 whole cell extract
Lane 2 : NCI-H929 nuclear extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 68 kDa7.5% SDS-PAGE gel.
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Immunoprecipitation - Anti-TCF3 / E2A antibody - N-terminal (ab229605)TCF3 / E2A was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with 4 μg ab229605. Western blot was performed from the immunoprecipitate using ab229605 at 1/500 dilution.
Lane 1: Jurkat whole cell extract 50 μg (Input).
Lane 2: 4 μg of preimmune Rabbit IgG instead of ab229605 in Jurkat whole cell extract.
Lane 3: ab229605 IP in Jurkat whole cell extract.
