Anti-TCF3 / E2A (phospho T355) antibody (ab85772)
Key features and details
- Rabbit polyclonal to TCF3 / E2A (phospho T355)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TCF3 / E2A (phospho T355) antibody
See all TCF3 / E2A primary antibodies -
Description
Rabbit polyclonal to TCF3 / E2A (phospho T355) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human TCF3/ E2A aa 350-450 conjugated to keyhole limpet haemocyanin.
(Peptide available asab103576) -
Positive control
- This antibody gave a positive signal in both HeLa and HepG2 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85772 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatHamsterDogApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 68 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 68 kDa).Target
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Function
Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. -
Involvement in disease
Note=Chromosomal aberrations involving TCF3 are cause of forms of pre-B-cell acute lymphoblastic leukemia (B-ALL). Translocation t(1;19)(q23;p13.3) with PBX1; Translocation t(17;19)(q22;p13.3) with HLF. Inversion inv(19)(p13;q13) with TFPT. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Domain
the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. -
Post-translational
modificationsPhosphorylated following NGF stimulation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6929 Human
- Entrez Gene: 21423 Mouse
- Entrez Gene: 171046 Rat
- Omim: 147141 Human
- SwissProt: P15923 Human
- SwissProt: P15806 Mouse
- SwissProt: P21677 Rat
- Unigene: 371282 Human
see all -
Alternative names
- AGM8 antibody
- bHLHb21 antibody
- Class B basic helix-loop-helix protein 21 antibody
see all
Images
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Western blot - Anti-TCF3 / E2A (phospho T355) antibody (ab85772)All lanes : Anti-TCF3 / E2A (phospho T355) antibody (ab85772) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human TCF3 / E2A peptide (ab118180) at 1 µg/ml
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Human TCF3 / E2A peptide (ab118180) at 1 µg/ml
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human TCF3 / E2A (phospho T355) peptide (ab103576) at 1 µg/ml
Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Human TCF3 / E2A (phospho T355) peptide (ab103576) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Datasheets and documents
References (0)
ab85772 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-TCF3 / E2A (phospho T355) antibody (ab85772)All lanes : Anti-TCF3 / E2A (phospho T355) antibody (ab85772) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human TCF3 / E2A peptide (ab118180) at 1 µg/ml
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Human TCF3 / E2A peptide (ab118180) at 1 µg/ml
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human TCF3 / E2A (phospho T355) peptide (ab103576) at 1 µg/ml
Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Human TCF3 / E2A (phospho T355) peptide (ab103576) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
