Anti-TCF3 / E2A antibody (ab228699)
Key features and details
- Rabbit polyclonal to TCF3 / E2A
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TCF3 / E2A antibody
See all TCF3 / E2A primary antibodies -
Description
Rabbit polyclonal to TCF3 / E2A -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseHuman
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Immunogen
Recombinant fragment within Human TCF3/ E2A (internal sequence). The exact sequence is proprietary.
Database link: P15923 -
Positive control
- WB: Jurkat, BCL-1 and NCI-H929 whole cell lysate. IP: Jurkat whole cell extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TCF3 / E2A antibody (ab228699)All lanes : Anti-TCF3 / E2A antibody (ab228699) at 1/5000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : NCI-H929 (human lymphoblastoid cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 67 kDa7.5% SDS-PAGE
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Immunoprecipitation - Anti-TCF3 / E2A antibody (ab228699)TCF3 / E2A was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract with 4 μg ab228699. Western blot was performed from the immunoprecipitate using ab228699 at 1/1000 dilution.
Lane 1: Jurkat whole cell extract 35 μg (Input).
Lane 2: Control IgG IP in Jurkat whole cell extract.
Lane 3: ab228699 IP in Jurkat whole cell extract.
7.5% SDS-PAGE
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Western blot - Anti-TCF3 / E2A antibody (ab228699)Anti-TCF3 / E2A antibody (ab228699) at 1/2000 dilution + BCL-1 (mouse lymphoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 67 kDa7.5% SDS-PAGE
