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Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper HLH

Anti-TCF3 / E2A antibody [7H3] - BSA and Azide free (ab252255)

Price and availability

526 012 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-TCF3 / E2A antibody [7H3] - BSA and Azide free (ab252255)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [7H3] to TCF3 / E2A - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat

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Overview

  • Product name

    Anti-TCF3 / E2A antibody [7H3] - BSA and Azide free
    See all TCF3 / E2A primary antibodies
  • Description

    Rat monoclonal [7H3] to TCF3 / E2A - BSA and Azide free
  • Host species

    Rat
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: WEHI-231 whole cell lysate. IHC-P: Mouse and rat spleen tissue.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab252255 is the carrier-free version of ab243152. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    7H3
  • Isotype

    IgG2b
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Nuclear
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF3 / E2A antibody [7H3] - BSA and Azide free (ab252255)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF3 / E2A antibody [7H3] - BSA and Azide free (ab252255)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling TCF3 / E2A with ab243152 at 5.37µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on mouse spleen tissue is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).
    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab243152).

  • Western blot - Anti-TCF3 / E2A antibody [7H3] - BSA and Azide free (ab252255)
    Western blot - Anti-TCF3 / E2A antibody [7H3] - BSA and Azide free (ab252255)
    All lanes : Anti-TCF3 / E2A antibody [7H3] (ab243152) at 0.537 µg/ml

    Lane 1 : C2C12 (mouse myoblasts myoblast), whole cell lysate
    Lane 2 : WEHI-231 (mouse B cell lymphoma B lymphocyte), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 67 kDa
    Observed band size: 67 kDa



    Exposure times: Lane 1: 3 minutes; Lane 2: 10 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab243152).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF3 / E2A antibody [7H3] - BSA and Azide free (ab252255)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF3 / E2A antibody [7H3] - BSA and Azide free (ab252255)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling TCF3 / E2A with ab243152 at 5.37µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on rat spleen tissue is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).
    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab243152).

  • Anti-TCF3 / E2A antibody [7H3] - BSA and Azide free (ab252255)
    Anti-TCF3 / E2A antibody [7H3] - BSA and Azide free (ab252255)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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