Anti-TCF3 / E2A antibody [7H3] (ab243152)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [7H3] to TCF3 / E2A
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-TCF3 / E2A antibody [7H3]
See all TCF3 / E2A primary antibodies -
Description
Rat monoclonal [7H3] to TCF3 / E2A -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseRatWB Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: WEHI-231 whole cell lysate. IHC-P: Mouse and rat spleen tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
7H3 -
Isotype
IgG2b -
Research areas
Images
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Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling TCF3 / E2A with ab243152 at 1/500 dilution, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on mouse spleen tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
All lanes : Anti-TCF3 / E2A antibody [7H3] (ab243152) at 0.537 µg/ml
Lane 1 : C2C12 (mouse myoblasts myoblast), whole cell lysate
Lane 2 : WEHI-231 (mouse B cell lymphoma B lymphocyte), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution
Predicted band size: 67 kDa
Observed band size: 67 kDaExposure times: Lane 1: 3 minutes; Lane 2: 10 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling TCF3 / E2A with ab243152 at 1/500 dilution, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on rat spleen tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -