Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µg 321 638 ₸ 10 µg 110 563 ₸

Order now and get it on Wednesday March 10, 2021

Anti-TCF3 / E2A antibody [7H3] (ab243152)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rat monoclonal [7H3] to TCF3 / E2A
Suitable for: IHC-P, WB
Reacts with: Mouse, Rat

Product name

Anti-TCF3 / E2A antibody [7H3]
See all TCF3 / E2A primary antibodies
Description

Rat monoclonal [7H3] to TCF3 / E2A
Host species

Rat
Tested Applications & Species

Application Species
IHC-P
Mouse

Rat

WB
Mouse

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: WEHI-231 whole cell lysate. IHC-P: Mouse and rat spleen tissue.
General notes
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
IHC-P	Mouse Rat
WB	Mouse

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

7H3
Isotype

IgG2b
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF3 / E2A antibody [7H3] (ab243152)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling TCF3 / E2A with ab243152 at 1/500 dilution, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on mouse spleen tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-TCF3 / E2A antibody [7H3] (ab243152)

All lanes : Anti-TCF3 / E2A antibody [7H3] (ab243152) at 0.537 µg/ml

Lane 1 : C2C12 (mouse myoblasts myoblast), whole cell lysate
Lane 2 : WEHI-231 (mouse B cell lymphoma B lymphocyte), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

Predicted band size: 67 kDa
Observed band size: 67 kDa

Exposure times: Lane 1: 3 minutes; Lane 2: 10 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF3 / E2A antibody [7H3] (ab243152)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling TCF3 / E2A with ab243152 at 1/500 dilution, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on rat spleen tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Anti-TCF3 / E2A antibody [7H3] (ab243152)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com