ID2 overexpression 293T lysate (whole cell) (ab94214)
Overview
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Product name
ID2 overexpression 293T lysate (whole cell) -
General notes
ab94214 is a 293T cell transfected lysate in which Human ID2 has been transiently over-expressed using a pCMV-ID2 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Developmental stage: Found in most early fetal tissues but not in the corresponding mature tissues. Function: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression. Similarity: Contains 1 basic helix-loop-helix (bHLH) domain. Tissue specificity: Highly expressed in early fetal tissues, including those of the central nervous system.
Images
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SDS-PAGE - ID2 overexpression 293T lysate (whole cell) (ab94214)ab94214 at 15μg/lane on an SDS-PAGE gel.
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Western blot - ID2 overexpression 293T lysate (whole cell) (ab94214)All lanes : Anti-ID2 antibody (ab89530) at 1/500 dilution
Lane 1 :ID2 overexpression 293T lysate (whole cell) (ab94214)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugate at 1/2500 dilution
