Antibodies, Proteins, Kits and Reagents for Life Science

351 792 ₸ Product size

100 µl 351 792 ₸ 10 µl 144 067 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Myogenin antibody [EPR4789] (ab124800)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4789] to Myogenin
Suitable for: ICC, WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Myogenin antibody [EPR4789]
See all Myogenin primary antibodies
Description

Rabbit monoclonal [EPR4789] to Myogenin
Host species

Rabbit
Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. This antibody has low affinity on skeletal muscle samples in WB. Please increase protein or antibody amount to get satisfied results.

Tested Applications & Species

Application	Species
ICC	Mouse
IHC-P	Human
WB	Mouse

See all applications and species data

Immunogen

Synthetic peptide within Human Myogenin. The exact sequence is proprietary.
Positive control
- WB: C2C12 and Human fetal muscle lysates; IHC-P: Human rhabdomyosarcoma tissue; ICC: ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612).
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4789
Isotype

IgG
Research areas

Immunocytochemistry - Anti-Myogenin antibody [EPR4789] (ab124800)

Immunofluorescence staining of Myogenin using ab124800 in ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612), which were differentiated for 3 days post induction.

The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab124800 at 0.5 µg/mL and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin, at 1/1000 dilution. Cells were then incubated with ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. Gamma is adjusted to 1.5 in all channels.

The antibody ab124800 gave comparable results using MeOH fixation (100%, 5 min).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myogenin antibody [EPR4789] (ab124800)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human rhabdomyosarcoma tissue sections labeling Myogenin with Purified ab124800 at 1:500 dilution (1.08 µg/ml). Heat mediated antigen retrieval was performed using using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
Western blot - Anti-Myogenin antibody [EPR4789] (ab124800)

All lanes : Anti-Myogenin antibody [EPR4789] (ab124800) at 0.5 µg/ml (purified)

Lane 1 : Human fetal skeletal muscle lysates
Lane 2 : Rat fetal skeletal muscle lysates
Lane 3 : Mouse fetal skeletal muscle lysates
Lane 4 : C2C12 (Mouse myoblasts myoblast) whole cell lysates
Lane 5 : C2C12 (Mouse myoblasts myoblast) treated with 2% horse serum whole cell lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 25 kDa

Blocking and diluting buffer: 5% NFDM/TBST.

This antibody has low affinity on skeletal muscle samples. Please increase protein or antibody amount to get satisfied results.
Myogenin expression is upregulated in C2C12 cells during differentiation process induced by horse serum treatment (PMID: 26563778).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myogenin antibody [EPR4789] (ab124800)

Unpurified ab124800, at a 1/100 dilution, staining Myogenin in paraffin embedded Human rhabdomyosarcoma tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Anti-Myogenin antibody [EPR4789] (ab124800)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com