Anti-Myogenin antibody [F5D] - BSA and Azide free (ab264517)
![Anti-Myogenin antibody [F5D] - BSA and Azide free (ab264517)](https://www.abcam.com/ps/products/264/ab264517/Images/ab264517-356287-ab1835339376ICCAP448139701.jpg "Anti-Myogenin antibody [F5D] - BSA and Azide free (ab264517)")
Key features and details
- Mouse monoclonal [F5D] to Myogenin - BSA and Azide free
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG1
Overview
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Product name
Anti-Myogenin antibody [F5D] - BSA and Azide free
See all Myogenin primary antibodies -
Description
Mouse monoclonal [F5D] to Myogenin - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Mouse
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Immunogen
Recombinant full length protein corresponding to Rat Myogenin aa 1-250.
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Positive control
- Rhabdomyosarcoma ICC/IF: 2 days differentiated C2C12 cells
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General notes
ab264517 is the carrier-free version of ab1835.If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
F5D -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Myogenin antibody [F5D] - BSA and Azide free (ab264517)
ab1835 staining Myogenin in undifferentiated C2C12 cells (top panel) and 2 days differentiated C2C12 cells (bottom panel).
The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab1835 at 1 μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution overnight at 4ºC. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab1835).
