Mouse Neutrophil Elastase ELISA Kit (ab252356)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 27.39 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Culture Media
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Neutrophil Elastase ELISA Kit
See all Neutrophil Elastase kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 2.6% Inter-assay Sample n Mean SD CV% Serum 3 3.2% -
Sample type
Cell culture supernatant, Serum, Tissue Culture Media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
27.39 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 107 104% - 112% Serum 108 99% - 116% Tissue Extracts 87 84% - 92% Hep Plasma 97 89% - 105% EDTA Plasma 96 88% - 105% Cit plasma 87 84% - 94% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow -
Product overview
Mouse Neutrophil Elastase ELISA Kit (ab252356) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Neutrophil Elastase protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Neutrophil Elastase with 27.39 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Neutrophil Elastase Capture Antibody 1 x 600µl 10X Mouse Neutrophil Elastase Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Mouse Neutrophil Elastase Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. -
Tissue specificity
Bone marrow cells. -
Involvement in disease
Defects in ELANE are a cause of cyclic haematopoiesis (CH) [MIM:162800]; also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency.
Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1) [MIM:202700]. SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. -
Sequence similarities
Belongs to the peptidase S1 family. Elastase subfamily.
Contains 1 peptidase S1 domain. - Information by UniProt
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Alternative names
- Bone marrow serine protease
- ELA2
- ELANE
see all -
Database links
- Entrez Gene: 50701 Mouse
- SwissProt: Q3UP87 Mouse
- Unigene: 262194 Mouse
Images
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Other - Mouse Neutrophil Elastase ELISA Kit (ab252356)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse Neutrophil Elastase standard curve in Sample Diluent NS.The Neutrophil Elastase standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse Neutrophil Elastase standard curve in 1X Cell Extraction Buffer PTR.The Neutrophil Elastase standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Neutrophil Elastase in mouse serum and plasma samples.The concentrations of Neutrophil Elastase were measured in duplicates, interpolated from the Neutrophil Elastase standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 40%, plasma (EDTA) 10%, and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neutrophil Elastase concentration was determined to be 32.45 ng/mL in serum, 6.06 ng/mL in plasma (citrate) and 15.17 ng/mL in plasma (EDTA) and 17.69 ng/mL plasma (heparin).
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Interpolated concentrations of native Neutrophil Elastase in mouse cell and tissue culture supernatant samples.The concentrations of Neutrophil Elastase were measured in duplicates, interpolated from the Neutrophil Elastase standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC cell culture supernatant 100% and lung tissue culture supernatant 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neutrophil Elastase concentration was determined to be 500.61 pg/mL in PBMC supernatant and 20.83 ng/mL in lung supernatant.
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Interpolated concentrations of native Neutrophil Elastase in mouse stomach extract (1000 ug/mL) and bone marrow extract (0.5 ug/mL) based on sample load.The concentrations of Neutrophil Elastase were measured in duplicate and interpolated from the Neutrophil Elastase standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neutrophil Elastase concentration was determined to be 2.02 ng/mL in stomach extract and 3.78 ng/mL in bone marrow extract.
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Sandwich ELISA - Mouse Neutrophil Elastase ELISA Kit (ab252356)To learn more about the advantages of recombinant antibodies see here.
