Human BMP9 ELISA Kit (ab272470)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.97 pg/ml
- Range: 4.69 pg/ml - 300 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human BMP9 ELISA Kit
See all BMP9 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.2% Inter-assay Sample n Mean SD CV% Serum 3 3.4% -
Sample type
Cell culture supernatant, Serum, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.97 pg/ml -
Range
4.69 pg/ml - 300 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 105 102% - 109% Serum 112 105% - 118% Cit plasma 102 97% - 105% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human BMP9 ELISA kit (ab272470) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human BMP9 protein in serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human BMP9 with 0.97 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Bone morphogenetic protein 9 (BMP9), also known as growth differentiation factor 2, is a protein encoded by the GDF2 gene and expressed in the liver. BMP9 is a member of the TGF-β superfamily that plays a role in angiogenesis as a potent inhibitor as well as a potent regulator for osteogenesis, glucose metabolism, and vascular tone. Studies have shown that mutations in BMP9 cause vascular disorders. Mouse, rat, cow, monkey share 96.4%, 94.5%, 92.7%, and 97.3% sequence homology, respectively.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human BMP9 Capture Antibody 1 x 600µl 10X Human BMP9 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human BMP9 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
BMP9 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. They are regulators of cell growth and differentiation. BMP9 has a role in the maturation of BFCN (basal forebrain cholinergic neurons) also inducing and maintaining the ability of these cells to respond to acetylcholine; BFCN are important for the processes of learning, memory and attention. BMP9 is a potent inducer of hepcidin (a cationic peptide that has a antimicrobial properties) in hepatocytes and can regulate iron metabolism. The physiological receptor of BMP9 is thought to be activin receptor-like kinase 1, ALK1 (also called ACVRL1), an endothelial-specific type I receptor of the TGF-beta receptor family. BMP9 is one of the most potent BMPs to induce orthotopic bone formation in vivo. BMP3, a blocker of most BMPs seems not to affect BMP9. -
Cellular localization
Secreted -
Alternative names
- BMP 9
- Bone morphogenetic protein 9
- GDF 2
see all -
Database links
- Entrez Gene: 2658 Human
- Omim: 605120 Human
- SwissProt: Q9UK05 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human BMP9 standard curve in Sample Diluent NS.The BMP9 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native BMP9 in human serum, plasma and cell culture supernatant samples.The concentrations of BMP9 were measured in duplicates, interpolated from the BMP9 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 50%, and HepG2 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BMP9 concentration was determined to be 213.4 pg/mL in serum, 166.3 pg/mL in plasma (citrate) and 37.3 pg/mL in HepG2 cell culture supernatant.
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Sandwich ELISA - Human BMP9 ELISA Kit (ab272470)To learn more about the advantages of recombinant antibodies see here.
