Anti-MyoD1 antibody [5.2F] (ab16148)
![Anti-MyoD1 antibody [5.2F] (ab16148)](https://www.abcam.com/ps/products/16/ab16148/Images/ab16148-24867-anti-myod1-antibody-52f-immunohistochemistry.jpg "Anti-MyoD1 antibody [5.2F] (ab16148)")
Key features and details
- Mouse monoclonal [5.2F] to MyoD1
- Suitable for: Sandwich ELISA, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG2a
Overview
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Product name
Anti-MyoD1 antibody [5.2F]
See all MyoD1 primary antibodies -
Description
Mouse monoclonal [5.2F] to MyoD1 -
Host species
Mouse -
Specificity
This antibody does not cross react with Myogenin, Myf5, or Myf6. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumansELISA Mouse
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Immunogen
Synthetic peptide corresponding to Mouse MyoD1 aa 3-56 (N terminal).
Sequence:LLSPPLRDIDLTGPDGSLCSFETADDFYDDPCFDSPDLRFFEDLDPRLVH VGAL
Database link: P10085 -
Positive control
- WB: Mouse muscle tissue lysate. ICC/IF: Rat differentiated skeletal muscle cells. IHC-P: Human rhabdomyosarcoma.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.08% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
5.2F -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG2a -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [5.2F] (ab16148)
Immunohistochemical staining using ab16148 at 2ug/ml on formalin fixed, paraffin embedded samples of human rhabdomyosarcoma.
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![Sandwich ELISA - Anti-MyoD1 antibody [5.2F] (ab16148)](https://www.abcam.com/ps/products/16/ab16148/Images/ab16148-24868-anti-myod1-antibody-52f-sandwich-elisa.jpg)
Sandwich ELISA - Anti-MyoD1 antibody [5.2F] (ab16148)Standard Curve for Myo-D; dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [5.2F] to MyoD1 (ab16148) at 5ug/ml and Detector Antibody Rabbit polyclonal to MyoD1 (ab64159) at 0.1ug/ml.
