Anti-MyoD1 antibody (ab64159)
Key features and details
- Rabbit polyclonal to MyoD1
- Suitable for: Sandwich ELISA, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MyoD1 antibody
See all MyoD1 primary antibodies -
Description
Rabbit polyclonal to MyoD1 -
Host species
Rabbit -
Tested applications
Suitable for: Sandwich ELISA, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow, Pig, Rhesus monkey
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human MyoD1.
Read Abcam's proprietary immunogen policy (Peptide available as ab66473.) -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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sELISA pair antibody
Applications
Our Abpromise guarantee covers the use of ab64159 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes Sandwich ELISA Use a concentration of 0.1 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [5.2F] to MyoD1 (ab16148). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [5.2F] to MyoD1 (ab16148) as Capture. WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa). Target
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Function
Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Post-translational
modificationsAcetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.
Ubiquitinated on the N-terminus; which is required for proteasomal degradation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 281938 Cow
- Entrez Gene: 4654 Human
- Entrez Gene: 17927 Mouse
- Entrez Gene: 407604 Pig
- Omim: 159970 Human
- SwissProt: Q7YS82 Cow
- SwissProt: P15172 Human
- SwissProt: P10085 Mouse
see all -
Alternative names
- bHLHc1 antibody
- Class C basic helix-loop-helix protein 1 antibody
- MYF 3 antibody
see all
Images
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Western blot - Anti-MyoD1 antibody (ab64159)All lanes : Anti-MyoD1 antibody (ab64159) at 1 µg/ml
Lane 1 : Skeletal Muscle (Mouse) Tissue Lysate at 20 µg
Lane 2 : Rh30 Whole Cell Lysate at 5 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 47 kDa (possible post-translational modification)
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab64159 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
ab64159 detects a band at 45 kDa, while this differs to its predicted molecular weight of 34kDa, the banding pattern observed is consistent with what has been described in the literature PMID:19352326.
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Sandwich ELISA - Anti-MyoD1 antibody (ab64159)Standard Curve for Myo-D; dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [5.2F] to MyoD1 (ab16148) at 5ug/ml and Detector Antibody Rabbit polyclonal to MyoD1 (ab64159) at 0.1ug/ml.
Datasheets and documents
References (16)
ab64159 has been referenced in 16 publications.
- Sato T et al. Core Transcription Factors Promote Induction of PAX3-Positive Skeletal Muscle Stem Cells. Stem Cell Reports 13:352-365 (2019). PubMed: 31353225
- Zheng Z et al. CDKN2B upregulation prevents teratoma formation in multipotent fibromodulin-reprogrammed cells. J Clin Invest 129:3236-3251 (2019). PubMed: 31305260
- Preussner J et al. Oncogenic Amplification of Zygotic Dux Factors in Regenerating p53-Deficient Muscle Stem Cells Defines a Molecular Cancer Subtype. Cell Stem Cell 23:794-805.e4 (2018). PubMed: 30449715
- Zhang P et al. Salidroside Inhibits Myogenesis by Modulating p-Smad3-Induced Myf5 Transcription. Front Pharmacol 9:209 (2018). PubMed: 29593538
- Mota R et al. Increasing Cardiomyocyte Atrogin-1 Reduces Aging-Associated Fibrosis and Regulates Remodeling in Vivo. Am J Pathol 188:1676-1692 (2018). PubMed: 29758183
Images
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Western blot - Anti-MyoD1 antibody (ab64159)All lanes : Anti-MyoD1 antibody (ab64159) at 1 µg/ml
Lane 1 : Skeletal Muscle (Mouse) Tissue Lysate at 20 µg
Lane 2 : Rh30 Whole Cell Lysate at 5 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 47 kDa (possible post-translational modification)
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab64159 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
ab64159 detects a band at 45 kDa, while this differs to its predicted molecular weight of 34kDa, the banding pattern observed is consistent with what has been described in the literature PMID:19352326.
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Sandwich ELISA - Anti-MyoD1 antibody (ab64159)Standard Curve for Myo-D; dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [5.2F] to MyoD1 (ab16148) at 5ug/ml and Detector Antibody Rabbit polyclonal to MyoD1 (ab64159) at 0.1ug/ml.
