Anti-TCF-4 antibody (ab130014)
Key features and details
- Rabbit polyclonal to TCF-4
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TCF-4 antibody
See all TCF-4 primary antibodies -
Description
Rabbit polyclonal to TCF-4 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Horse, Cow, Dog, Macaque monkey, Gorilla, Orangutan
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Immunogen
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Positive control
- This antibody gave a positive signal in the following Mouse tissue lysates: Cortex; Cerebellum; Olfactory Bulb; Hippocampus; Liver; as well as the following whole cell lysates: HepG2; HCT116; Caco2.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab130014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa). Target
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Function
Transcription factor that binds to the immunoglobulin enchancer Mu-E5/KE5-motif. Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription (By similarity). Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'. -
Tissue specificity
Expressed in adult heart, brain, placenta, skeletal muscle and to a lesser extent in the lung. In developing embryonic tissues, expression mostly occurs in the brain. -
Involvement in disease
Defects in TCF4 are a cause of Pitt-Hopkins syndrome (PTHS) [MIM:610954]. PTHS is a rare syndromic encephalopathy characterized by severe psychomotor delay, epilepsy, daily bouts of diurnal hyperventilation starting in infancy, mild postnatal growth retardation, postnatal microcephaly, and distinctive facial features. Since most hitherto reported cases have been sporadic, with males and females equally affected, PTHS is regarded as an autosomal dominant condition. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Domain
the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 403949 Dog
- Entrez Gene: 6925 Human
- Entrez Gene: 21413 Mouse
- Omim: 602272 Human
- SwissProt: P15881 Dog
- SwissProt: P15884 Human
- SwissProt: Q60722 Mouse
- Unigene: 605153 Human
see all -
Alternative names
- bHLHb19 antibody
- Class B basic helix-loop-helix protein 19 antibody
- E2 2 antibody
see all
Images
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Western blot - Anti-TCF-4 antibody (ab130014)All lanes : Anti-TCF-4 antibody (ab130014) at 1 µg/ml
Lane 1 : Mouse Cortex Tissue Lysate
Lane 2 : Cerebellum Mouse Tissue Lysate
Lane 3 : Mouse Olfactory Bulb Lysate
Lane 4 : Mouse Hippocampus Tissue Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : Liver (Mouse) Tissue Lysate
Lane 7 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
Lane 8 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 71 kDa
Observed band size: 71 kDa
Additional bands at: 260 kDa, 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab130014 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
References (3)
ab130014 has been referenced in 3 publications.
- Zhou JY et al. Hydrolyzed wheat gluten alleviates deoxynivalenol-induced intestinal injury by promoting intestinal stem cell proliferation and differentiation via upregulation of Wnt/ß-catenin signaling in mice. Food Chem Toxicol 131:110579 (2019). PubMed: 31202940
- Jung M et al. Analysis of the expression pattern of the schizophrenia-risk and intellectual disability gene TCF4 in the developing and adult brain suggests a role in development and plasticity of cortical and hippocampal neurons. Mol Autism 9:20 (2018). PubMed: 29588831
- Rathert P et al. Transcriptional plasticity promotes primary and acquired resistance to BET inhibition. Nature 525:543-7 (2015). PubMed: 26367798
Images
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Western blot - Anti-TCF-4 antibody (ab130014)All lanes : Anti-TCF-4 antibody (ab130014) at 1 µg/ml
Lane 1 : Mouse Cortex Tissue Lysate
Lane 2 : Cerebellum Mouse Tissue Lysate
Lane 3 : Mouse Olfactory Bulb Lysate
Lane 4 : Mouse Hippocampus Tissue Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : Liver (Mouse) Tissue Lysate
Lane 7 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
Lane 8 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 71 kDa
Observed band size: 71 kDa
Additional bands at: 260 kDa, 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab130014 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
