Anti-DDX23 antibody (ab228999)
Key features and details
- Rabbit polyclonal to DDX23
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-DDX23 antibody
See all DDX23 primary antibodies -
Description
Rabbit polyclonal to DDX23 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Rhesus monkey
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Immunogen
Recombinant fragment within Human DDX23. The exact sequence is proprietary.
Database link: Q9BUQ8 -
Positive control
- WB: HeLa, NIH-3T3, JC whole cell lysates. IHC-P: HBL435 xenograft. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DDX23 antibody (ab228999)All lanes : Anti-DDX23 antibody (ab228999) at 1/1000 dilution
Lane 1 : NIH-3T3 (mouse embryo fibroblast cell line) whole cell lysate.
Lane 2 : JC (Mouse mammary gland cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 96 kDa7.5% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-DDX23 antibody (ab228999)Paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma cell line) cells stained for DDX23 protein using ab228999 at 1/500 dilution in ICC/IF.
Alpha-tubulin filaments (red).
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Western blot - Anti-DDX23 antibody (ab228999)Anti-DDX23 antibody (ab228999) at 1/1000 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 96 kDa7.5% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX23 antibody (ab228999)Paraffin-embedded HBL435 xenograft tissue stained for DDX23 protein using ab228999 at 1/500 dilution in immunohistochemical analysis.
