Anti-STAT2 (phospho Y690) antibody (ab53132)
Key features and details
- Rabbit polyclonal to STAT2 (phospho Y690)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-STAT2 (phospho Y690) antibody
See all STAT2 primary antibodies -
Description
Rabbit polyclonal to STAT2 (phospho Y690) -
Host species
Rabbit -
Specificity
ab53132 detects endogenous levels of STAT2 only when phosphorylated at tyrosine 690. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human STAT2. A synthetic phosphopeptide derived from human STAT2 around the phosphorylation site of tyrosine 690 (R-K-YP-L-K).
Database link: P52630 -
Positive control
- IHC-P: Rat kidney, Human colon. WB: HeLa cells treated with IFN.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab53132 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-STAT2 (phospho Y690) antibody (ab53132)All lanes : Anti-STAT2 (phospho Y690) antibody (ab53132) at 1/300 dilution
Lane 1 : HeLa cell extract treated with IFN (2500U/ml, 30mins)
Lane 2 : HeLa cell extract treated with IFN (2500U/ml, 30mins) with immunising peptide
Predicted band size: 98 kDa
Observed band size: 98 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 (phospho Y690) antibody (ab53132)ab53132, at 1/50 dilution, staining STAT2 in paraffin embedded human lung carcinoma tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising peptide.
