Anti-STAT2 antibody - C-terminal (ab227312)
Key features and details
- Rabbit polyclonal to STAT2 - C-terminal
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-STAT2 antibody - C-terminal
See all STAT2 primary antibodies -
Description
Rabbit polyclonal to STAT2 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human STAT2 (C terminal). The exact sequence is proprietary.
Database link: P52630 -
Positive control
- WB: Jurkat, Raji, K562 and THP-1 whole cell lysates. IHC-P: Human cervical carcinoma and endometrial carcinoma tissues. IP: HEK-293T whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-STAT2 antibody - C-terminal (ab227312) at 1/2000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lane 4 : THP-1 (human monocytic leukemia cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 97 kDa5% SDS-PAGE gel.
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Paraffin-embedded human cervical carccinoma tissue staining STAT2 using ab227312 at 1/500 dilution in immunohistochemical analysis.
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STAT2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with 2 µg ab227312. Western blot was performed from the immunoprecipitate using ab227312 at 1/1000 dilution. Anti-rabbit IgG was used as secondary antibody.
Lane 1: HEK-293T whole cell lysate 40ug.
Lane 2: Control IP in HEK-293T whole cell lysate with 2 μg of preimmune rabbit IgG.
Lane 3: ab227312 IP in HEK-293T whole cell lysate.
7.5% SDS-PAGE gel.
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Paraffin-embedded human endrometrial carccinoma tissue staining STAT2 using ab227312 at 1/500 dilution in immunohistochemical analysis.