Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E208] to STAT5 (phospho Y694)
  • Suitable for: WB, IHC-P, ELISA
  • Reacts with: Human

Overview

  • Product name

    Anti-STAT5 (phospho Y694) antibody [E208]
    See all STAT5 primary antibodies

  • Description

    Rabbit monoclonal [E208] to STAT5 (phospho Y694)

  • Host species

    Rabbit

  • Specificity

    The antibody only detects Stat5 phosphorylated on Tyrosine 694.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human STAT5 (phospho Y694).

  • Positive control

    • WB: A431 cell lysate treated with epidermal growth factor. IHC-P: Human breast carcinoma tissue.

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)
    Western blot - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)
    All lanes : Anti-STAT5 (phospho Y694) antibody [E208] (ab32364) at 1/1000 dilution (purified)

    Lane 1 : A431 cell lysate, untreated
    Lane 2 : A431 cell lysate, treated with Epidermal growth factor

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 90 kDa
    Observed band size: 90 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human infiltrating duct carcinoma of breast tissue labelling STAT5 (phospho Y694) with purified ab32364 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • ELISA - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)
    ELISA - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364) This image is courtesy of Hanna Dreja

    Serially diluted ab32364 was bound to immobilised phospho peptide (P) - or control peptides ((C), 1 microgram x mL-1). The antibody was detected by HRP-labelled goat anti-rabbit IgG (ab97080; diluted 1: 50,000) and signal was developed with TMB substrate

  • Western blot - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)
    Western blot - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)
    All lanes : Anti-STAT5 (phospho Y694) antibody [E208] (ab32364) at 1/1000 dilution (unpurified)

    Lane 1 : A431 cell lysate, untreated
    Lane 2 : A431 cell lysate, treated with EGF
    Lane 3 : A431 cell lysate, treated with phosphatase

    Predicted band size: 90 kDa
    Observed band size: 90 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma using unpurified ab32364 at a 1/50 dilution.

  • Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)
    Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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