Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-STAT5 (phospho Y694) antibody [E208] - BSA and Azide free (ab219607)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E208] to STAT5 (phospho Y694) - BSA and Azide free
  • Suitable for: WB, IHC-P, ELISA
  • Reacts with: Human

Overview

  • Product name

    Anti-STAT5 (phospho Y694) antibody [E208] - BSA and Azide free
    See all STAT5 primary antibodies

  • Description

    Rabbit monoclonal [E208] to STAT5 (phospho Y694) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The antibody only detects Stat5 phosphorylated on Tyrosine 694.

  • Tested applications

    Suitable for: WB, IHC-P, ELISAmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A431 cell lysate treated with epidermal growth factor. IHC-P: Human breast carcinoma tissue.

  • General notes

    ab219607 is the carrier-free version of ab32364 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab219607 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • ELISA - Anti-STAT5 (phospho Y694) antibody [E208] - BSA and Azide free (ab219607)
    ELISA - Anti-STAT5 (phospho Y694) antibody [E208] - BSA and Azide free (ab219607) This image is courtesy of an Abreview submitted by Hanna Dreja.

    Serially diluted ab32364 was bound to immobilised phospho peptide (P) - or control peptides ((C), 1 microgram x mL-1). The antibody was detected by HRP-labelled goat anti-rabbit IgG (ab97080; diluted 1: 50,000) and signal was developed with TMB substrate

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32364).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 (phospho Y694) antibody [E208] - BSA and Azide free (ab219607)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 (phospho Y694) antibody [E208] - BSA and Azide free (ab219607)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human infiltrating duct carcinoma of breast tissue labelling STAT5 (phospho Y694) with purified ab32364 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32364).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 (phospho Y694) antibody [E208] - BSA and Azide free (ab219607)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 (phospho Y694) antibody [E208] - BSA and Azide free (ab219607)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma using unpurified ab32364 at a 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32364).

  • Anti-STAT5 (phospho Y694) antibody [E208] - BSA and Azide free (ab219607)
    Anti-STAT5 (phospho Y694) antibody [E208] - BSA and Azide free (ab219607)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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