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Signal Transduction Signaling Pathway Nuclear Signaling STATs

Anti-STAT5b antibody (ab235934)

Anti-STAT5b antibody (ab235934)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to STAT5b
  • Suitable for: ChIP, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-STAT5b antibody
    See all STAT5b primary antibodies
  • Description

    Rabbit polyclonal to STAT5b
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ChIP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig
  • Immunogen

    Recombinant fragment corresponding to Human STAT5b aa 1-300.
    Database link: P51692

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human breast cancer, placenta and testis tissue. ChIP: Chromatin prepared from MCF7 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • STATs
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • STATs
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b antibody (ab235934)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b antibody (ab235934)

    Paraffin-embedded human breast cancer tissue stained for STAT5b using ab235934 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b antibody (ab235934)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b antibody (ab235934)

    Paraffin-embedded human placenta tissue stained for STAT5b using ab235934 at 1/100 dilution in immunohistochemical analysis.

  • ChIP - Anti-STAT5b antibody (ab235934)
    ChIP - Anti-STAT5b antibody (ab235934)

    ChIP analysis on chromatin prepared from MCF7 (human breast adenocarcinoma cell line) cells. 1.1 x 106 cells were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4µg ab235934 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the AGT promoter.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b antibody (ab235934)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b antibody (ab235934)

    Paraffin-embedded human testis tissue stained for STAT5b using ab235934 at 1/100 dilution in immunohistochemical analysis.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-STAT5b antibody (ab235934)

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    Anti-STAT5b antibody (ab226193)

    Applications: IHC-P, IP, WB

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    Alexa Fluor® 647 Anti-STAT5b antibody [EPR16671] (ab199846)

    Applications: ICC/IF

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    Anti-STAT5b antibody [EPR16671] (ab178941)

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    Anti-STAT5b (phospho S731) antibody (ab52211)

    Applications: ICC/IF, IHC-P, WB

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    Alexa Fluor® 488 Anti-STAT5b antibody [EPR16671] (ab199767)

    Applications: Flow Cyt, ICC/IF

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    Anti-STAT5b antibody [EPR16671] - BSA and Azide free (ab240211)

    Applications: ChIP, Flow Cyt, ICC/IF, IHC-P, IP, WB

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