Anti-STAT5b (phospho S731) antibody (ab52211)
Key features and details
- Rabbit polyclonal to STAT5b (phospho S731)
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-STAT5b (phospho S731) antibody
See all STAT5b primary antibodies -
Description
Rabbit polyclonal to STAT5b (phospho S731) -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic phosphopeptide based on human STAT5B around the phosphorylation site of serine 731 (A-P-SP-P-A).
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General notes
ab52211 detects endogenous levels of STAT5B but only when phosphorylated at serine 731 (human) or serine 730 (mouse and rat).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
ab52211 detects endogenous levels of STAT5B but only when phosphorylated at serine 731 (human) or serine 730 (mouse and rat). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-STAT5b (phospho S731) antibody (ab52211)All lanes : Anti-STAT5b (phospho S731) antibody (ab52211) at 1/500 dilution
Lane 1 : Extracts from RAW264.7 cells
treated with EGF (200ng/ml, 30min)
Lane 2 : Extracts from RAW264.7 cells
treated with EGF (200ng/ml, 30min), ab52211 was pre-incubated with immunising (blocking) peptide
Predicted band size: 90 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b (phospho S731) antibody (ab52211)Paraffin-embedded human breast carcinoma tissue stained for STAT5b (phospho S731), using ab52211 (1/100). The right hand panel represents a negative control where ab52211 was pre-incubated with the immunising (blocking) peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-STAT5b (phospho S731) antibody (ab52211)ICC/IF image of ab52211 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52211, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
